Portal de Periódicos da CAPES

Tau Phosphorylation Patterns in the Rat Cerebral Cortex After Traumatic Brain Injury and Sodium Selenate Effects: An Epibios4rx Project 2 Study

2023; Mary Ann Liebert, Inc.; Volume: 41; Issue: 1-2 Linguagem: Inglês

10.1089/neu.2022.0219

1557-9042

Patricia G. Saletti, Pablo M. Casillas‐Espinosa, Christos Panagiotis Lisgaras, Wenzhu Mowrey, Qianyun Li, Wei Liu, Rhys D. Brady, Idrish Ali, Juliana Silva, Glenn R. Yamakawa, Matt Hudson, Crystal Li, Emma L. Braine, Lisa D. Coles, James C. Cloyd, Nigel C. Jones, Sandy R. Shultz, Solomon L. Moshé, Terence J. O’Brien, Aristea S. Galanopoulou,

Mitochondrial Function and Pathology

Sodium selenate (SS) activates protein phosphatase 2 (PP2A) and reduces phosphorylated tau (pTAU) and late post-traumatic seizures after lateral fluid percussion injury (LFPI). In EpiBioS4Rx Project 2, a multi-center international study for post-traumatic targets, biomarkers, and treatments, we tested the target relevance and modification by SS of pTAU forms and PP2A and in the LFPI model, at two sites: Einstein and Melbourne. In Experiment 1, adult male rats were assigned to LFPI and sham (both sites) and naïve controls (Einstein). Motor function was monitored by neuroscores. Brains were studied with immunohistochemistry (IHC), Western blots (WBs), or PP2A activity assay, from 2 days to 8 weeks post-operatively. In Experiment 2, LFPI rats received SS for 7 days (SS0.33: 0.33 mg/kg/day; SS1: 1 mg/kg/day, subcutaneously) or vehicle (Veh) post-LFPI and pTAU, PR55 expression, or PP2A activity were studied at 2 days and 1 week (on treatment), or 2 weeks (1 week off treatment). Plasma selenium and SS levels were measured. In Experiment 1 IHC, LFPI rats had higher cortical pTAU-Ser