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State of the Art Procedures for the Isolation and Characterization of Mesoangioblasts

2023; Springer Science+Business Media; Linguagem: Inglês

10.1007/978-1-0716-3036-5_8

1940-6029

Nefele Giarratana, Filippo Conti, Lorenza Rinvenuti, Flavio Ronzoni, Maurilio Sampaolesi,

Tissue Engineering and Regenerative Medicine

Adult skeletal muscle is a dynamic tissue able to regenerate quite efficiently, thanks to the presence of stem cell machinery. Besides the quiescent satellite cells that are activated upon injury or paracrine factors, other stem cells are described to be directly or indirectly involved in adult myogenesis. Mesoangioblasts (MABs) are vessel-associated stem cells originally isolated from embryonic dorsal aorta and, at later stages, from the adult muscle interstitium expressing pericyte markers. Adult MABs entered clinical trials for the treatment of Duchenne muscular dystrophy and the transcriptome of human fetal MABs has been described. In addition, single cell RNA-seq analyses provide novel information on adult murine MABs and more in general in interstitial muscle stem cells. This chapter provides state-of-the-art techniques to isolate and characterize murine MABs, fetal and adult human MABs.