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Comparative transcriptome-based identification and expression analysis of ATP-binding cassette (ABC) transporters reveals a likely role in the transport of <i>β</i>-caryophyllene and response to abiotic stress in <i>Brassica campestris</i>

2023; Volume: 3; Issue: 1 Linguagem: Inglês

10.48130/vr-2023-0013

2769-0520

Haibin Wang, Chen Zong, Yibo Bai, Shuilin Yuan, Yan Li, Tiantian Han, Huanhuan Xu, Chun‐Mei Hu, Xilin Hou, Ying Li,

Nitrogen and Sulfur Effects on Brassica

β -caryophyllene has physiological activities of antibacterial, antiedemic, anti-inflammatory, antitumor, and fungicidal activities. Previous research has focused on the synthesis of β -caryophyllene. However, little information is available about its involvement in β -caryophyllene transport. ATP-binding cassette (ABC) transporters are involved in the transport of multiple substrates such as amino acids, terpenes, and heavy metals. Herein, we present a genome-wide comparative transcriptome analysis between non- β -caryophyllene cultivars ('SZQ') and high β -caryophyllene cultivars ('XQC') to identify potential ABC functional gene activities that occur during plant development. This article analyzes potential ABC transporters that may transport β -caryophyllene by comparative transcriptome and GC-MS, and explores the genetic structure, evolutionary relationship, cis-acting element analysis, and transcription patterns of these transporters under different treatment conditions. Phylogenetic and cis-acting element analysis indicated that 8 genes in 'SZQ' and 'XQC' belonged to the ABCC, ABCG, and ABCE subfamilies and had the highest number of light-responsive elements and MYB binding sites. The RNA-seq and qRT-PCR results showed that 8 BcABC genes were expressed in a tissue- and development-specific manner, while their abundance apparently varied when plants were exposed to different treatment conditions. Pearson correlation analysis showed that ABCG18 is a potential carrier of β -caryophyllene, which might participate in substance transport in leaves and petioles. In summary, these observations opened up new future research directions for β -caryophyllene transport and provided insight into ABC family genes in Brassica campestris .