pOpsicle: An all-optical reporter system for synaptic vesicle recycling combining pH-sensitive fluorescent proteins with optogenetic manipulation of neuronal activity
2023; Frontiers Media; Volume: 17; Linguagem: Inglês
10.3389/fncel.2023.1120651
ISSN1662-5102
AutoresMarius Seidenthal, Barbara Jánosi, Nils Rosenkranz, Noah Schuh, Nora Elvers, Miles Willoughby, Xinda Zhao, Alexander Gottschalk,
Tópico(s)Lipid Membrane Structure and Behavior
ResumopH-sensitive fluorescent proteins are widely used to study synaptic vesicle (SV) fusion and recycling. When targeted to the lumen of SVs, fluorescence of these proteins is quenched by the acidic pH. Following SV fusion, they are exposed to extracellular neutral pH, resulting in a fluorescence increase. SV fusion, recycling and acidification can thus be tracked by tagging integral SV proteins with pH-sensitive proteins. Neurotransmission is generally activated by electrical stimulation, which is not feasible in small, intact animals. Previous
Referência(s)