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Phytase production by white-rot fungi cultivated in liquid medium with particulate agro-industrial byproducts

2023; Matthias-Grünewald-Verlag; Volume: 23; Issue: 5 Linguagem: Inglês

10.53660/clm-1075-23c74

0010-5236

Aline Maria Pereira Batistela, Marisangela Isabel Wietzikoski Halabura, Katielle Vieira Avelino, Kariny Aparecida Jardim Rúbio, Daniela Dib Gonçalves, Giani Andréa Linde, Nelson Barros Colauto, Maria Graciela Iecher Faria, Juliana Silveira do Valle,

Pineapple and bromelain studies

This study aimed to screen phytase-producing basidiomycetes by submerged cultivation (SmC) with particulate agro-industrial byproducts and evaluate the enzyme's thermal and pH stability. Five among eight fungal species produced high phytase activity (> 300 U mL-1) after seven days in SmC. Ganoderma sp., the best phytase producer (1130 U mL-1), was grown in a liquid medium with particulate agro-industrial byproducts (coffee husks, citric pulp, sugarcane bagasse, and soybean hulls). Soybean hulls increased phytase activity (16%) and productivity (2.3-fold). The residual phytase activity was determined after 120 min incubation in different pH (pH 3, 5, 7, and 9) and temperatures (30, 50, and 70 oC). Phytase activity remained stable at pH ranging from 3 to 9, with 100% activity retention after 120 min at pH 7. Furthermore, Phytase retained more than 60% activity when incubated for 120 min from 30 oC to 70 oC. Our findings support future studies using Ganoderma sp. and particulate soybean hulls for phytase production for potential use in the animal feed industry.