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Incidence of Begomovirus and Crinivirus in tomato and potato crops in Paraná State, Brazil

2023; UNIVERSIDADE FEDERAL DE SANTA MARIA; Volume: 53; Issue: 11 Linguagem: Inglês

10.1590/0103-8478cr20210883

1678-4596

Evandro Keller, Gabriel Koch, Mayara Catherine Candido Silva, Patrícia Wyler, J. C. Barbosa, Jorge Alberto Marques Rezende, Henrique da Silva Silveira Duarte, Renata Faier Calegario,

Cocoa and Sweet Potato Agronomy

ABSTRACT: In the last decades, the high incidence of viruses transmitted by whiteflies has become a problem in the tomato fields, threatening, more recently, the potato crops. The present study carried out a survey of begomoviruses and criniviruses in tomato and potato crops, from 2015 to 2018, in the municipalities of Araucária, Campo do Tenente, Campo Largo, Contenda, Lapa, Faxinal, Morretes, Reserva, Castro, Palmeira and São Mateus do Sul, in Paraná State, Brazil. Total DNA and RNA from leaves were extracted and used as templates to detect, respectively, begomoviruses by PCR and criniviruses by RT-PCR. Out of 215 tomato samples, 14 from Faxinal were infected by crinivirus. The other tomato samples and 243 potato samples showed negative results for begomovirus and crinivirus. Results indicated a low incidence (6.5%) of crinivirus infecting tomato crops in Paraná State, and the nucleotide sequence of one amplified fragment shared 99.71% identity with tomato chlorotic virus (ToCV).