Portal de Periódicos da CAPES

Artigo Acesso aberto Produção Nacional Revisado por pares

BIBTEX RIS

Antigen-Specific Antibody Signature Is Associated with COVID-19 Outcome

2023; Multidisciplinary Digital Publishing Institute; Volume: 15; Issue: 4 Linguagem: Inglês

10.3390/v15041018

1999-4915

Bárbara Batista Salgado, Maele Ferreira Jordão, Thiago Barros do Nascimento de Morais, Danielle Severino Sena da Silva, Ivanildo Vieira Pereira Filho, Wlademir Braga Salgado Sobrinho, Nani Oliveira Carvalho, Rafaella Oliveira dos Santos, Julia Forato, Priscilla P. Barbosa, Daniel A. Toledo-Teixeira, Kerollen Runa Pinto, Ingrid Silva Correia, Isabelle Bezerra Cordeiro, Júlio Nino de Souza Neto, Enedina Nogueira de Assunção, Fernando Val, Gisely Cardoso de Melo, Vanderson de Souza Sampaio, Wuelton Marcelo Monteiro, Fabiana Granja, William Marciel de Souza, Spartaco Astolfi Filho, José Luiz Proença‐Módena, Jaila Dias Borges Lalwani, Marcus Lacerda, Paulo Afonso Nogueira, Pritesh Lalwani,

Long-Term Effects of COVID-19

Numerous studies have focused on inflammation-related markers to understand COVID-19. In this study, we performed a comparative analysis of spike (S) and nucleocapsid (N) protein-specific IgA, total IgG and IgG subclass response in COVID-19 patients and compared this to their disease outcome. We observed that the SARS-CoV-2 infection elicits a robust IgA and IgG response against the N-terminal (N1) and C-terminal (N3) region of the N protein, whereas we failed to detect IgA antibodies and observed a weak IgG response against the disordered linker region (N2) in COVID-19 patients. N and S protein-specific IgG1, IgG2 and IgG3 response was significantly elevated in hospitalized patients with severe disease compared to outpatients with non-severe disease. IgA and total IgG antibody reactivity gradually increased after the first week of symptoms. Magnitude of RBD-ACE2 blocking antibodies identified in a competitive assay and neutralizing antibodies detected by PRNT assay correlated with disease severity. Generally, the IgA and total IgG response between the discharged and deceased COVID-19 patients was similar. However, significant differences in the ratio of IgG subclass antibodies were observed between discharged and deceased patients, especially towards the disordered linker region of the N protein. Overall, SARS-CoV-2 infection is linked to an elevated blood antibody response in severe patients compared to non-severe patients. Monitoring of antigen-specific serological response could be an important tool to accompany disease progression and improve outcomes.