Produção Nacional
Revisado por pares
Can SARS‐CoV ‐2 screening in oral biopsies aid epidemiological surveillance?
2023; Wiley; Volume: 52; Issue: 7 Linguagem: Inglês
10.1111/jop.13438
ISSN1600-0714
AutoresRoberta Rayra Martins‐Chaves, Marina Gonçalves Diniz, Lucyene Miguita, Fernanda Aragão Felix, F. Rocha, Paula Luize Camargos Fonseca, Victor Emmanuel Viana Geddes, Diego Menezes, Rennan Garcias Moreira, Tatiana Nayara Libório‐Kimura, Jeconias Câmara, Tássia Caroline da Costa Mendes, Hélder Antônio Rebelo Pontes, Flávia Sirotheau Corrêa Pontes, Thayanne Oliveira de Freitas Gonçalves, Thamyres Campos Fonsêca, Aline Corrêa Abrahão, Mário José Romañach, Ana Paula Negreiros Nunes Alves, Karuza Maria Alves Pereira, Danyel Elias da Cruz Pérez, Elaine Judite de Amorim Carvalho, Jean Nunes dos Santos, Flávia Caló de Aquino Xavier, Fernando Costa Giffoni, Alessandra Hübner de Souza, Carolina Cavaliéri Gomes, Sílvia Ferreira de Sousa, Felipe Paiva Fonseca, Renan P. Souza, Renato Santana Aguiar, Ricardo Santiago Gomez,
Tópico(s)SARS-CoV-2 and COVID-19 Research
ResumoAbstract Background Three years after the first confirmed COVID‐19 case in Brazil, the outcomes of Federal government omissions in managing the crisis and anti‐science stance heading into the pandemic have become even more evident. With over 36 million confirmed cases and nearly 700 000 deaths up to January 2023, the country is one of the hardest‐hit places in the world. The lack of mass‐testing programs was a critical broken pillar responsible for the quick and uncontrolled SARS‐CoV‐2 spread throughout the Brazilian population. Faced with this situation, we aimed to perform the routine SARS‐CoV‐2 screening through RT‐qPCR of oral biopsies samples to aid in the asymptomatic epidemiological surveillance during the principal outbreak periods. Methods We analyzed 649 formalin‐fixed paraffin‐embedded oral tissue samples from five important oral and maxillofacial pathology laboratories from the north, northeast, and southeast geographic regions of Brazil. We also sequenced the whole viral genome of positive cases to investigate SARS‐CoV‐2 variants. Results The virus was detected in 9/649 analyzed samples, of which three harbored the Variant of Concern Alpha (B.1.1.7). Conclusion Although our approach did not value aiding asymptomatic epidemiological surveillance, we could successfully identify a using FFPE tissue samples. Therefore, we suggest using FFPE tissue samples from patients who have confirmed diagnosis of SARS‐CoV‐2 infection for phylogenetic reconstruction and contraindicate the routine laboratory screening of these samples as a tool for asymptomatic epidemiological surveillance.
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