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Cowanin induces apoptosis in breast cancer cells via Bcl-2 signaling pathway

2023; De Gruyter; Volume: 20; Issue: 3 Linguagem: Inglês

10.1515/jcim-2023-0058

2194-6329

Fatma Sri Wahyuni, Suryati Syafri, Dita Permatasari, Dira Hefni, Dachriyanus Dachriyanus, Nur Azizah Nomiza, Indri Aulia Rezti,

Plant-Derived Bioactive Compounds

This study aimed to investigate the effect of cowanin the mechanism of cowanin toward cell death and BCL-2 protein (antiapoptotic) expression of T47D breast cancer.The cell death was evaluated by double staining, namely acridine orange and propidium iodide, and then observed under a fluorescence microscope. Meanwhile, the BCL-2 protein expression was determined by western blotting with measurement of protein area and protein density.The result found T47D breast cancer cells were viable, apoptosis, and necrosis after treatment with cowanin. The average viable cells, apoptosis, and necrosis percentages were 54.13 %, 45.43 %, and 0.44 %, respectively. Statistical analysis showed cowanin could significantly induce death in T47D breast cancer cells by apoptosis (p<0.05). It was also revealed that cowanin and positive control (doxorubicin) treatment had a significantly decreased protein area and protein density (p<0.05).It can be concluded that cowanin can induce death in T47D breast cancer cells by apoptosis and affect the expression of Bcl-2 protein in T47D breast cancer cells.