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Cross-talk between phospholipid synthesis and peptidoglycan expansion by a cell wall hydrolase

2023; National Academy of Sciences; Volume: 120; Issue: 24 Linguagem: Inglês

10.1073/pnas.2300784120

1091-6490

Nilanjan Som, Manjula Reddy,

Protein Structure and Dynamics

The Gram-negative bacterial cell envelope is a complex multilayered structure comprising a bilayered phospholipid (PL) membrane that surrounds the cytoplasm (inner membrane or IM) and an asymmetric outer membrane (OM) with PLs in the inner leaflet and lipopolysaccharides in the outer leaflet. Between these two layers is the periplasmic space, which contains a highly cross-linked mesh-like glycan polymer, peptidoglycan (PG). During cell expansion, coordinated synthesis of each of these components is required to maintain the integrity of the cell envelope; however, it is currently not clear how such coordination is achieved. In this study, we show that a cross-link-specific PG hydrolase couples the expansion of PG sacculus with that of PL synthesis in the Gram-negative model bacterium,