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Balancing read length and sequencing depth: Optimizing Nanopore long‐read sequencing for monocots with an emphasis on the Liliales

2023; Botanical Society of America; Volume: 11; Issue: 3 Linguagem: Inglês

10.1002/aps3.11524

2168-0450

Gisel Y. De La Cerda, Jacob B. Landis, Evan Eifler, Adriana I. Hernández, Fay‐Wei Li, Jing Zhang, Carrie M. Tribble, Nisa Karimi, Patricia P. Chan, Thomas J. Givnish, Susan R. Strickler, Chelsea D. Specht,

Chromosomal and Genetic Variations

We present approaches used to generate long-read Nanopore sequencing reads for the Liliales and demonstrate how modifications to standard protocols directly impact read length and total output. The goal is to help those interested in generating long-read sequencing data determine which steps may be necessary for optimizing output and results.