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Longitudinal and quantitative fecal shedding dynamics of SARS-CoV-2, pepper mild mottle virus, and crAssphage

2023; American Society for Microbiology; Volume: 8; Issue: 4 Linguagem: Inglês

10.1128/msphere.00132-23

2379-5042

Peter J. Arts, J. Daniel Kelly, Claire M. Midgley, Khamal Anglin, Scott Lu, Glen R. Abedi, Raul Andino, Kevin M. Bakker, Bryon Banman, Alexandria B. Boehm, Melissa Briggs‐Hagen, Andrew F. Brouwer, Michelle C. Davidson, Marisa C. Eisenberg, Miguel Garcia-Knight, Sterling Knight, Michael J. Peluso, Jesus Pineda-Ramirez, Ruth Diaz Sanchez, Sharon Saydah, Michel Tassetto, Jeffrey N. Martin, Krista R. Wigginton,

Clostridium difficile and Clostridium perfringens research

Wastewater-based epidemiology (WBE) emerged during the coronavirus disease 2019 (COVID-19) pandemic as a scalable and broadly applicable method for community-level monitoring of infectious disease burden. The lack of high-resolution fecal shedding data for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) limits our ability to link WBE measurements to disease burden. In this study, we present longitudinal, quantitative fecal shedding data for SARS-CoV-2 RNA, as well as for the commonly used fecal indicators pepper mild mottle virus (PMMoV) RNA and crAss-like phage (crAssphage) DNA. The shedding trajectories from 48 SARS-CoV-2-infected individuals suggest a highly individualized, dynamic course of SARS-CoV-2 RNA fecal shedding. Of the individuals that provided at least three stool samples spanning more than 14 days, 77% had one or more samples that tested positive for SARS-CoV-2 RNA. We detected PMMoV RNA in at least one sample from all individuals and in 96% (352/367) of samples overall. CrAssphage DNA was detected in at least one sample from 80% (38/48) of individuals and was detected in 48% (179/371) of all samples. The geometric mean concentrations of PMMoV and crAssphage in stool across all individuals were 8.7 × 10