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Organized B cell sites in cartilaginous fishes reveal the evolutionary foundation of germinal centers

2023; Cell Press; Volume: 42; Issue: 7 Linguagem: Inglês

10.1016/j.celrep.2023.112664

2639-1856

Hanover Matz, Richard S. Taylor, Anthony K. Redmond, Thomas Hill, Rose Ruiz Daniels, Mariana Beltrán, Neil C. Henderson, Daniel J. Macqueen, Helen Dooley,

Atherosclerosis and Cardiovascular Diseases

The absence of germinal centers (GCs) in cartilaginous fishes lies at odds with data showing that nurse sharks can produce robust antigen-specific responses and affinity mature their B cell repertoires. To investigate this apparent incongruity, we performed RNA sequencing on single nuclei, allowing us to characterize the cell types present in the nurse shark spleen, and RNAscope to provide in situ cellular resolution of key marker gene expression following immunization with R-phycoerythrin (PE). We tracked PE to the splenic follicles where it co-localizes with CXCR5