Portal de Periódicos da CAPES

Molecular Analysis of Breast Cancer BRCA1 and BRCA2 in Southern Punjab

2022; Volume: 22; Issue: 3 Linguagem: Inglês

10.19080/ctoij.2022.22.556088

2473-554X

Muhammad Waqar Mazhar,

Molecular Biology Techniques and Applications

estimated 52% of families, to BRCA2 in 32% of families, and to neither of them in 16%.The majority of families with male and female breast cancer were due to BRCA2 (76%) [7]. Material and Methods FamiliesBreast cancer families were ascertained at MINAR in Multan, Pakistan from June 2018 to December 2018.80 Breast cancer samples were collected.They were classified into 7 categories based on family history: Group A1: Families with female breast cancer diagnosed under 40 years of age.Group A2: Families with female breast cancer diagnosed 41 -60 year of age.Group A3: Families with female breast cancer diagnosed above 61 years of age.This study was carried out on breast cancer in female belong to the southern Punjab (from district Burrywala, Layyah, Khanewal, chichawatni, D.G Khan, Bahawalpur, Multan, Vehari, Muzaffargarh, Bakkhar and Sahiwal) Pakistan. DNA IsolationGenomic DNA was extracted from 20 ml heparin blood samples using the Favorgen PrepTM DNA Extraction Kit, according to manufacturer's instruction.The PCR reaction was as follows: 95 oC for 4 min, 95oC for 1mint, 49oC for 30 sec, 72oC for 1min, 35 cycles, followed by a 5 min final extension at the 72 oC.Amplified BRCA1 and BRCA2 DNA was further checked on 1.5 % agarose gel for the resolution of different band patterns.