Produção Nacional
Revisado por pares
First Report Worldwide of Fusarium sororula Associated with Pineapple that Causes Fruit Rot
2023; American Phytopathological Society; Volume: 107; Issue: 7 Linguagem: Inglês
10.1094/pdis-08-22-1837-pdn
ISSN1943-7692
AutoresTarciana Silva dos Santos, Márcio Akio Ootani, Iwanne Lima Coelho, Wilza Carla Oliveira de Souza, Luciana Cordeiro do Nascimento, Thaís Regina Pintino De Almeida, Fabiana Aparecida Cavalcante Silva, Igor Alexsander De Melo Pimentel, Marcelo Garcia De Oliveira, Delson Laranjeira,
Tópico(s)Agricultural and Food Sciences
ResumoHomePlant DiseaseVol. 107, No. 7First Report Worldwide of Fusarium sororula Associated with Pineapple that Causes Fruit Rot PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report Worldwide of Fusarium sororula Associated with Pineapple that Causes Fruit RotTarciana Silva Dos Santos, Marcio Akio Ootani, Iwanne Lima Coelho, Wilza Carla Oliveira De Souza, Luciana Cordeiro Do Nascimento, Thaís Regina Pintino De Almeida, Fabiana Silva, Igor Alexsander De Melo Pimentel, Marcelo Garcia De Oliveira, and Delson LaranjeiraTarciana Silva Dos Santos†Corresponding author: T. S. D. Santos; E-mail Address: [email protected]https://orcid.org/0000-0001-9713-8679Phytosanity Department, Federal Rural University of Pernambuco - UFRPE, Recife, Pernambuco, BrazilSearch for more papers by this author, Marcio Akio OotaniDepartment of Biotechnology, Federal University of Tocantins - UFT, Gurupi, Tocantins, BrazilSearch for more papers by this author, Iwanne Lima CoelhoPhytosanity Department, Federal Rural University of Pernambuco - UFRPE, Recife, Pernambuco, BrazilSearch for more papers by this author, Wilza Carla Oliveira De SouzaPhytosanity Department, Federal University of Paraíba - UFPB, Areia, Paraíba, BrazilSearch for more papers by this author, Luciana Cordeiro Do NascimentoPhytosanity Department, Federal University of Paraíba - UFPB, Areia, Paraíba, BrazilSearch for more papers by this author, Thaís Regina Pintino De Almeidahttps://orcid.org/0000-0003-2519-3710Phytosanity Department, Federal Rural University of Pernambuco - UFRPE, Recife, Pernambuco, BrazilSearch for more papers by this author, Fabiana SilvaCenter for Strategic Technologies of the Northeast - CETENE, Recife, Pernambuco, BrazilSearch for more papers by this author, Igor Alexsander De Melo PimentelPhytosanity Department, Federal Rural University of Pernambuco - UFRPE, Recife, Pernambuco, BrazilSearch for more papers by this author, Marcelo Garcia De OliveiraPhytosanity Department, Federal Rural University of Pernambuco - UFRPE, Recife, Pernambuco, BrazilSearch for more papers by this author, and Delson Laranjeirahttps://orcid.org/0000-0002-1895-1766Phytosanity Department, Federal Rural University of Pernambuco - UFRPE, Recife, Pernambuco, BrazilSearch for more papers by this authorAffiliationsAuthors and Affiliations Tarciana Silva Dos Santos1 † Marcio Akio Ootani2 Iwanne Lima Coelho1 Wilza Carla Oliveira De Souza3 Luciana Cordeiro Do Nascimento3 Thaís Regina Pintino De Almeida1 Fabiana Silva4 Igor Alexsander De Melo Pimentel1 Marcelo Garcia De Oliveira1 Delson Laranjeira1 1Phytosanity Department, Federal Rural University of Pernambuco - UFRPE, Recife, Pernambuco, Brazil 2Department of Biotechnology, Federal University of Tocantins - UFT, Gurupi, Tocantins, Brazil 3Phytosanity Department, Federal University of Paraíba - UFPB, Areia, Paraíba, Brazil 4Center for Strategic Technologies of the Northeast - CETENE, Recife, Pernambuco, Brazil Published Online:3 Jul 2023https://doi.org/10.1094/PDIS-08-22-1837-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articlePineapple (Ananas comosus L.) production generates substantial economic value in Brazilian agriculture (Azevedo et al. 2022), but it is significantly affected by Fusarium spp. In November 2014, fruit exudate symptoms were seen on Pearly pineapple plants in a production area in county Pombos, Pernambuco State, Northeast Brazil (8°08′21.2″S 35°23′49.9″W, altitude 208 m). The symptoms included spotted leaves, withering, and fruit with internal rotting, with the tissue having a soft consistency and dark brown coloring and exudate formation externally. Leaves and fruit were sanitized for 1 min in 1% NaOCl, washed twice in sterile water, cut into smaller pieces, plated on 10 sterile Petri plates with potato dextrose agar (PDA), and incubated at 26°C for 7 days in a biochemical oxygen demand chamber. Isolates morphologically similar to Fusarium spp. were purified from monosporically cultured colonies, with plates showing a slightly lilac color. On PDA medium, morphological characteristics of conidia were hyaline microconidia, ovoid or ellipsoid, and 1.0 to 3.0 × 1.0 to 2.0 μm. Macroconidia were elongate, ellipsoid, and slightly curved, 5.0 to 14.0 × 1.0 to 2.0 μm. These characteristics are descriptive of F. sororula (Herron et al. 2015). Ten isolates were selected, and DNA was extracted with DNA HIMEDIA. The PCR reactions were prepared using PCR Master Mix (PROMEGA) and were amplified with primer pairs for the genes β-tubulin (Btub2; 336 bp) and RNA polymerase II (Rpb2; 702 bp) and elongation factor 1 (Tef1; 580 bp) region. DNA from only one isolate, CMA23, was amplified, using 35 cycles of denaturation at 95°C for 30 s, followed by annealing at 59, 58, and 53°C, respectively, for 30 s, then extension at 72°C for 1.0 min, and final extension at 72°C for 10 min. The sequences were deposited in GenBank (accession nos. ON751963-1, ON456385.1, and MK316363.1) and compared to described Fusarium species. According to Yilmaz et al. (2021), several Fusarium species were identified using morphological and phylogenetic analyses based on the partial sequence described above, and they had 100% similarity to the Fusarium fujikuroi species complex (FFSC) (Herron et al. 2015). Based on a bootstrap test with 1,000 replications, we constructed phylogenetic trees using the maximum parsimony method. The clade of CMA23 was identified as F. sororula with 83% similarity. Ten Pearly pineapple fruits and 10 detached leaves were sanitized for pathogenicity tests, then perforated with a toothpick. A spore suspension of 5 μl at 1 × 106 spores ml−1 was added (four inoculation points/fruit and detached leaf). They were incubated for 12 days at 22 ± 5°C and 70 to 78% humidity and a 12-h photoperiod. Only fruits and leaves, injured with toothpicks immersed in spore suspension, showed necrotic spots on leaves, fruit rot, and exudate, the same symptoms as fruits found in the field. The fruits showed no symptoms when pierced with a toothpick in sterile water. The CMA23 isolate was molecularly identified using the same method described above, fulfilling Koch՚s postulates. Fusarium guttiforme was for a long time considered the only Fusarium disease in pineapple (Stepien et al. 2013). and was previously described as the causal agent of fusariosis on pineapple in Brazil. However, using molecular techniques, new species have been shown to be pathogens of this crop, mainly belonging to the FFSC, such as Fusarium proliferatum and Fusarium verticillioides (Ibrahim et al. 2017). This is the first report worldwide of F. sororula that causes fruit rot.The author(s) declare no conflict of interest.References:Azevedo, R. G., et al. 2022. Clean. Mater. 3:100040. https://doi.org/10.1016/j.clema.2021.100040 Crossref, Google ScholarHerron, D. A., et al. 2015. Stud. Mycol. 80:131. https://doi.org/10.1016/j.simyco.2014.12.001 Crossref, ISI, Google ScholarIbrahim, N. F., et al. 2017. J. Phytopathol. 165:718. https://doi.org/10.1111/jph.12611 Crossref, ISI, Google ScholarStepien, Ł., et al. 2013. J. Appl. Genet. 54:367. https://doi.org/10.1007/s13353-013-0146-0 Crossref, ISI, Google ScholarYilmaz, N., et al. 2021. Persoonia 46:129. https://doi.org/10.3767/persoonia.2021.46.05 ISI, Google ScholarFunding: Funding was provided by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 107, No. 7 July 2023SubscribeISSN:0191-2917e-ISSN:1943-7692 Download Metrics Article History Issue Date: 26 Jul 2023 Published: 3 Jul 2023 Accepted: 7 Dec 2022 Page: 2231 Information© 2023 The American Phytopathological SocietyFundingCoordenação de Aperfeiçoamento de Pessoal de Nível SuperiorKeywordsAnanas comosusfungusfusariosisnecrotic leaf spotThe author(s) declare no conflict of interest.PDF download
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