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“Relationship between antibiotic resistance, biofilm formation, virulence factors and source of origin of Pseudomonas aeruginosa environmental isolates with regard to the presence of metallo-β-lactamase-encoding genes”

2023; Elsevier BV; Volume: 182; Linguagem: Inglês

10.1016/j.micpath.2023.106223

1096-1208

Letícia Franco Gervasoni, Inaiá Calegari Peixoto, Anna Cardoso Imperador, Lívia Batista De Oliveira, Lucimeire Fernandes Correia, Karolinny Cristiny de Oliveira Vieira, Erika Kushikawa Saeki, Paula Esteves da Silva Lima, Edson Assunção Mareco, Valéria Cataneli Pereira, Lizziane Kretli Winkelströter Eller,

Oral microbiology and periodontitis research

A total of 557 water samples were evaluated and of these, 23 were positive for the presence of Pseudomonas aeruginosa. Approximately 91.7% of them were weak biofilm formers. Only 4 isolates showed antimicrobial resistance. All isolates presented Twitching motility, a positive result for the production of pyocyanin, alkaline protease, and hemolysins. The genotypic tests showed: lasA, (95.6%) lasB (95.6%), exoS (95.6%), exoT (91.3%), toxA (91.3%), akgO (91.3%), plcN (91.3%) aprA (86.9%), phzM (78.3%), and pvdA (60.9%). For genes encoding metallo-beta-lactamase, it was found: blaVIM (56.6%), blaSPM (4.3%), and blaSIM (47.8%). A strong association was found between the metallo-beta-lactamase producing genes, nine genes of virulence factors and the motility (r = 0.6231). The very close clonal profile suggests a probable similarity between the isolates from different cities. Thus, P. aeruginosa can be present in water supplies with variable virulence capacities and can generate a huge concern for human, animal, and environmental health.