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Proteomic Identification of Metabolic Changes in Paracoccidioides Brasiliensis Induced by a Nitroheteroarylchalcone

2023; Future Medicine; Volume: 18; Issue: 15 Linguagem: Inglês

10.2217/fmb-2022-0150

1746-0921

Marcos Antonio Batista de Carvalho Júnior, Lívia do Carmo Silva, Olívia Basso Rocha, Amanda Alves de Oliveira, Igor Godinho Portis, Antônio Alonso, Laís Alonso, Kleber SF e Silva, Marcelo do Nascimento Gomes, Carolina Horta Andrade, Célia MA Soares, Maristela Pereira,

Phytochemical compounds biological activities

Aim: To access the metabolic changes caused by a chalcone derivative (LabMol-75) through a proteomic approach. Methods: Proteomic analysis was performed after 9 h of Paracoccidioides brasiliensis yeast (Pb18) cell incubation with the LabMol-75 at MIC. The proteomic findings were validated through in vitro and in silico assays. Results: Exposure to the compound led to the downregulation of proteins associated with glycolysis and gluconeogenesis, β-oxidation, the citrate cycle and the electron transport chain. Conclusion:LabMol-75 caused an energetic imbalance in the fungus metabolism and deep oxidative stress. Additionally, the in silico molecular docking approach pointed to this molecule as a putative competitive inhibitor of DHPS.