Produção Nacional
Revisado por pares
First Report of Pectobacterium brasiliense Causing Soft Rot on Chicory ( Cichorium intybus subsp. intybus ) in Brazil
2023; American Phytopathological Society; Volume: 107; Issue: 7 Linguagem: Inglês
10.1094/pdis-08-22-1988-pdn
ISSN1943-7692
AutoresMariana Pereira Appy, Lucas Vitor, Bruna Isabele Bueno Macedo, Mariana Ferreira-Tonin, Ricardo Harakava, Suzete Aparecida Lanza Destéfano,
Tópico(s)Plant-Microbe Interactions and Immunity
ResumoHomePlant DiseaseVol. 107, No. 7First Report of Pectobacterium brasiliense Causing Soft Rot on Chicory (Cichorium intybus subsp. intybus) in Brazil PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of Pectobacterium brasiliense Causing Soft Rot on Chicory (Cichorium intybus subsp. intybus) in BrazilMariana Pereira Appy, Lucas Vitor, Bruna Isabele Bueno Macedo, Mariana Ferreira-Tonin, Ricardo Harakava, and Suzete Aparecida Destefano LanzaMariana Pereira AppyLaboratory of Plant Bacteriology, Instituto Biológico, Campinas 13101-680, SP, BrazilSearch for more papers by this author, Lucas VitorLaboratory of Plant Bacteriology, Instituto Biológico, Campinas 13101-680, SP, BrazilSearch for more papers by this author, Bruna Isabele Bueno MacedoLaboratory of Plant Bacteriology, Instituto Biológico, Campinas 13101-680, SP, BrazilSearch for more papers by this author, Mariana Ferreira-ToninLaboratory of Plant Bacteriology, Instituto Biológico, Campinas 13101-680, SP, BrazilSearch for more papers by this author, Ricardo HarakavaLaboratory of Phytopathological Biochemistry, Instituto Biológico, São Paulo 04014-002, SP, BrazilSearch for more papers by this author, and Suzete Aparecida Destefano Lanza†Corresponding author: S. A. Destefano Lanza; E-mail Address: [email protected]https://orcid.org/0000-0002-7535-9224Laboratory of Plant Bacteriology, Instituto Biológico, Campinas 13101-680, SP, BrazilSearch for more papers by this authorAffiliationsAuthors and Affiliations Mariana Pereira Appy1 Lucas Vitor1 Bruna Isabele Bueno Macedo1 Mariana Ferreira-Tonin1 Ricardo Harakava2 Suzete Aparecida Destefano Lanza1 † 1Laboratory of Plant Bacteriology, Instituto Biológico, Campinas 13101-680, SP, Brazil 2Laboratory of Phytopathological Biochemistry, Instituto Biológico, São Paulo 04014-002, SP, Brazil Published Online:13 Jul 2023https://doi.org/10.1094/PDIS-08-22-1988-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleChicory (Cichorium intybus subsp. intybus) is one of Brazil’s most popular vegetables in production and consumption. It is part of a produce sector that generates a gross domestic product of more than six billion dollars and provides approximately two million jobs. In 2017, the Laboratory of Plant Bacteriology of the Instituto Biológico (Campinas, SP, Brazil) received chicory plants from Cabreúva, São Paulo state, with symptoms of soft rot. There were water-soaked areas with necrotic regions throughout the plant tissue from stems to leaves. Infected leaf tissue showed irregular, brown to black lesions 12 to 14 mm in diameter surrounded by yellowish haloes. Abundant bacterial streaming was observed from lesions under an optical microscope at 100×. The bacterium isolated from lesions was deposited as IBSBF 3375 in the Phytobacteria Culture Collection of Instituto Biológico (IBSBF) (Campinas, SP, Brazil, http://www.biologico.sp.gov.br/page/colecoes/fitobacterias), registered at the World Federation of Culture Collections. The strain formed white to cream, glistening, convex colonies on nutrient agar. It was facultatively anaerobic with gram-negative rods and nonfluorescent on King’s medium B. It elicited a hypersensitive response in tobacco plants and a pectolytic response on crystal violet pectate agar and in an assay with potato slices. The genomic DNA of the isolate was extracted according to Pitcher et al. (1989) for molecular analyses. The 16S-23S intergenic transcribed spacer (ITS) region and rpoB gene were amplified by PCR using the methods of Toth et al. (2001) and Ferreira-Tonin et al. (2012), respectively. Both amplicons were digested using restriction enzymes CfoI and RsaI (PCR-RFLP) using four different Pectobacterium type strains as controls (P. chrysanthemi IBSBF 231, P. carotovorum subsp. carotovorum IBSBF 863, P. odoriferum IBSBF 1814, and P. brasiliense IBSBF 1692). The results showed an identical restriction profile to P. brasiliense for both amplicons. To further validate identification, the specific primers BR1f/L1r were used for PCR, and a predicted amplicon of 322 bp expected for P. brasiliense was produced (Duarte et al. 2004). The 16S rRNA sequence of strain IBSBF 3375 was deposited in GenBank (ON568308) and showed >99% similarity to that of P. brasiliense (syn. P. carotovorum subsp. brasiliensis, Pcb) strains DFLB4 (1,006/1,008 bp; OM909040.1) and SJDR (1,002/1,004 bp; OM3333246.1). Pathogenicity was tested to fulfill Koch’s postulates by spraying bacterial inoculum (108 CFU/ml) on leaves of 40-day-old plants. Forty chicory plants were used in the treatments: without bacterial inoculation (sterilized distilled water as a negative control), P. odoriferum IBSBF 1814 (species already reported in chicory, used as a positive control), P. brasiliense IBSBF 1692 (type strain), and strain IBSBF 3375, under greenhouse conditions. Seven days after inoculation, all plants inoculated with bacteria showed water-soaked areas with necrotic symptoms, while negative control samples remained healthy. The bacteria were reisolated from the IBSBF 3375 inoculated tissues and identified as the original pathogen by the molecular techniques already described. P. brasiliense is a very aggressive pathogen, with several reports in crops; it has been reported to cause soft rot on diverse plant hosts, including sweet potato, radish, and kale (Boluk et al. 2020; Liu et al. 2019; Voronina et al. 2019). This study showed that Pcb has more host plants that need to be investigated, highlighting a need to survey for pectinolytic bacteria to apply management strategies to reduce losses in the Brazilian vegetable market. To our knowledge, this is the first report of P. brasiliense causing soft rot on chicory in Brazil.The author(s) declare no conflict of interest.References:Boluk, G., et al. 2020. Plant Dis. 104:2721. https://doi.org/10.1094/PDIS-04-20-0701-PDN Link, ISI, Google ScholarDuarte, V., et al. 2004. J. Appl. Microbiol. 96:535. https://doi.org/10.1111/j.1365-2672.2004.02173.x Crossref, ISI, Google ScholarFerreira-Tonin, M., et al. 2012. Int. J. Syst. Evol. Microbiol. 62:1419. https://doi.org/10.1099/ijs.0.028977-0 Crossref, ISI, Google ScholarLiu, H., et al. 2019. Plant Dis. 103:1409. https://doi.org/10.1094/PDIS-11-18-2107-PDN Link, ISI, Google ScholarPitcher, D. G., et al. 1989. Lett. Appl. Microbiol. 8:151. https://doi.org/10.1111/j.1472-765X.1989.tb00262.x Crossref, ISI, Google ScholarToth, I. K., et al. 2001. Appl. Envi Microbiol. 67:4070. https://doi.org/10.1128/AEM.67.9.4070-4076.2001 Crossref, ISI, Google ScholarVoronina, M. V., et al. 2019. Plant Dis. 103:364. https://doi.org/10.1094/PDIS-03-18-0456-PDN Link, ISI, Google ScholarThe author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 107, No. 7 July 2023SubscribeISSN:0191-2917e-ISSN:1943-7692 Download Metrics Article History Issue Date: 26 Jul 2023Published: 13 Jul 2023Accepted: 7 Nov 2022 Page: 2214 Information© 2023 The American Phytopathological SocietyKeywordsfield croppathogen detectionprokaryotesvegetablesThe author(s) declare no conflict of interest.PDF download
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