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BIBTEX RIS

Shotgun Metagenomic Sequencing Reveals Virome Composition of Mosquitoes from a Transition Ecosystem of North-Northeast Brazil

2023; Multidisciplinary Digital Publishing Institute; Volume: 14; Issue: 7 Linguagem: Inglês

10.3390/genes14071443

2073-4425

Carine Fortes Aragão, Sandro Patroca da Silva, Bruna Laís Sena do Nascimento, Fábio Silva da Silva, Joaquim Pinto Nunes Neto, Valéria Cristina Soares Pinheiro, Ana Cecília Ribeiro Cruz,

Plant Virus Research Studies

A wide diversity of pathogenic mosquito-borne viruses circulate in the Brazilian Amazon, and the intense deforestation can contribute to the spread of these viruses. In this context, this study aimed to investigate the viral diversity in mosquitoes of the genera Aedes, Culex, Haemagogus, and Sabethes from a transition area between the Amazon, Cerrado, and Caatinga biomes in Brazil. Metagenomic high-throughput sequencing was used to characterize the virome of 20 mosquito pools. A total of 15 virus-like genomes were identified, comprising species genomically close to insect-specific viruses of the families Iflaviridae, Metaviridae, Lispiviridae, Rhabdoviridae, Xinmoviridae, and Parvoviridae and species of plant viruses of the families Solemoviridae, Virgaviridae, and Partitiviridae. However, sequences of viruses associated with human and animal diseases were not detected. Most of the recovered genomes were divergent from those previously described. These findings reveal that there are a large number of unknown viruses to be explored in the middle-north of Brazil.