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Two novel protein kinase C-related genes of fission yeast are essential for cell viability and implicated in cell shape control.

1993; Springer Nature; Volume: 12; Issue: 5 Linguagem: Inglês

10.1002/j.1460-2075.1993.tb05848.x

1460-2075

Takashi Toda, Mizuki Shimanuki, Mitsuhiro Yanagida,

Genomics and Chromatin Dynamics

Research Article1 May 1993free access Two novel protein kinase C-related genes of fission yeast are essential for cell viability and implicated in cell shape control. T. Toda T. Toda Department of Biophysics, Faculty of Science, Kyoto University, Japan. Search for more papers by this author M. Shimanuki M. Shimanuki Department of Biophysics, Faculty of Science, Kyoto University, Japan. Search for more papers by this author M. Yanagida M. Yanagida Department of Biophysics, Faculty of Science, Kyoto University, Japan. Search for more papers by this author T. Toda T. Toda Department of Biophysics, Faculty of Science, Kyoto University, Japan. Search for more papers by this author M. Shimanuki M. Shimanuki Department of Biophysics, Faculty of Science, Kyoto University, Japan. Search for more papers by this author M. Yanagida M. Yanagida Department of Biophysics, Faculty of Science, Kyoto University, Japan. Search for more papers by this author Author Information T. Toda1, M. Shimanuki1 and M. Yanagida1 1Department of Biophysics, Faculty of Science, Kyoto University, Japan. The EMBO Journal (1993)12:1987-1995https://doi.org/10.1002/j.1460-2075.1993.tb05848.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Two novel protein kinase C (PKC)-like genes, pck1+ and pck2+ were isolated from fission yeast by PCR. Both contain common domains of PKC-related molecules, but lack a putative Ca(2+)-binding domain so that they may belong to the nPKC group. Gene disruption of pck1+ and pck2+ establishes that they share an overlapping essential function for cell viability. Cells of a single pck2 deletion display severe defects in cell shape; they are irregular and sometimes pear-like instead of cylindrical. In contrast, the induced overexpression of pck2+ is lethal, producing multiseptated and branched cells. These results suggest that fission yeast PKC-like genes are involved in the polarity of cell growth control. We show that pck2 is allelic to sts6, a locus we have previously identified by its supersensitivity to staurosporine, a potent protein kinase inhibitor [Toda et al. (1991) Genes Dev., 5, 60–73]. In addition, the lethal overexpression of pck2+ can be suppressed by staurosporine, indicating that fission yeast pck1 and pck2 are molecular targets of this inhibitor. Previous ArticleNext Article Volume 12Issue 51 May 1993In this issue RelatedDetailsLoading ...