Portal de Periódicos da CAPES

Artigo Produção Nacional Revisado por pares

BIBTEX RIS

Synergistic Effect of Hydralazine Associated with Triazoles on Candida spp. in Planktonic Cells

2023; Future Medicine; Volume: 18; Issue: 10 Linguagem: Inglês

10.2217/fmb-2023-0012

1746-0921

Francisca Bruna Stefany Aires do Nascimento, Lívia Gurgel do Amaral Valente Sá, João Batista de Andrade Neto, Letícia Serpa Sampaio Moreno, Helaine Almeida Queiroz, Lisandra Juvêncio da Silva, Vitória Pessoa de Farias Cabral, Daniel Sampaio Rodrigues, Sidnei Samuel Pereira, Bruno C. Cavalcanti, Jacilene Silva, Emmanuel Silva Marinho, Hélcio Silva dos Santos, Manoel Odorico de Moraes, Hélio Vitoriano Nobre Júnior, Cecília Rocha da Silva,

Phenothiazines and Benzothiazines Synthesis and Activities

Objective: To evaluate the antifungal activity of hydralazine hydrochloride alone and in synergy with azoles against Candida spp. and the action mechanism. Methods: We used broth microdilution assays to determine the MIC, checkerboard assays to investigate synergism, and flow cytometry and molecular docking tests to ascertain action mechanism. Results: Hydralazine alone had antifungal activity in the range of 16-128 μg/ml and synergistic effect with itraconazole versus 100% of the fungal isolates, while there was synergy with fluconazole against 11.11% of the isolates. There was molecular interaction with the receptors exo-B(1,3)-glucanase and CYP51, causing reduced cell viability and DNA damage. Conclusion: Hydralazine is synergistic with itraconazole and triggers cell death of Candida spp. at low concentrations, demonstrating antifungal potential.