Portal de Periódicos da CAPES

Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA

2023; Cell Press; Volume: 186; Issue: 19 Linguagem: Inglês

10.1016/j.cell.2023.08.025

1097-4172

Yasuyuki Hosono, Yashar S. Niknafs, John R. Prensner, Matthew K. Iyer, Saravana M. Dhanasekaran, Rohit Mehra, Sethuramasundaram Pitchiaya, Jean C. Tien, June Escara‐Wilke, Anton Poliakov, Shih‐Chun Chu, Sahal Saleh, Keerthana Sankar, Fengyun Su, Shuling Guo, Yuanyuan Qiao, Susan M. Freier, Huynh‐Hoa Bui, Xuhong Cao, Rohit Malik, Timothy M. Johnson, David G. Beer, Felix Y. Feng, Weibin Zhou, Arul M. Chinnaiyan,

Molecular Biology Techniques and Applications

(Cell 171, 1559–1572.e1–e20; December 14, 2017) In the original Figure 4E, nucleotide numbers shown in the schematic representation of THOR were incorrect. The positions labeled as 143 and 196 should be 196 and 388, respectively. The corrected Figure 4E is shown below. Also, in the original figure legend of Figure S2, the order of the figure description was incorrect. The alphabetical labels (E), (F), (G), (H), (I), (J), and (K) should be (K), (J), (E), (F), (G), (H), and (I), respectively. The corrected legend of Figure S2 is shown below. The authors regret these errors and apologize for any confusion they may have caused. Figure S2. Characterization of THOR Transcript, Coding Potential, and Tissue Expression, Related to Figure 1 (A) Northern blot of endogenous THOR in H1299 cells, and of H1437 cells expressing LacZ control, THOR, and THOR with the addition of siRNA targeting THOR. Blot of gapdh provided as a control. (B) Bar plot depicting the qPCR expression of the long versus short THOR isoform. (C) qPCR expression of the long THOR isoform following addition of siRNA. (D) Northern blot of THOR in zebrafish kidney and testis. Blot of GAPDH provided as a control. (E) Coding probability scores for the transcripts were assessed by Coding Potential Assessment Tool (CPAT). NRAS and TP53 used as positive control, and SCHLAP1 as a negative control. (F) Coding probability scores for the PhyloCSF and CPC tools for THOR and MYC. Values less than 0 suggest a lack of coding potential. (G) Genome browser depiction of the THOR locus with aggregate ribosomal profiling track (red), aggregate poly-A RNA-seq track (green) and GENCODE v22 genome annotation obtained from the GWIPS-viz ribo-seq genome browser. (H) H&E image of the testis and surrounding tissue architecture. (I) H&E (left) and THOR ISH (right) for the human testis, rete, and adipose. (J) 3’ RACE for the THOR transcripts expressed by the lentiviral system. PCR agarose gel (left) shows two bands utilized in Sanger sequencing (right). (K) 5’ RACE for the THOR transcripts expressed by the lentiviral system. PCR agarose gel (left) confirms single band used in Sanger sequencing (right). Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNAHosono et al.CellDecember 14, 2017In BriefAn ultraconserved lncRNA promotes oncogenesis. Full-Text PDF Open Archive