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Stieleria tagensis sp. nov., a novel member of the phylum Planctomycetota isolated from Tagus River in Portugal

2023; Springer Science+Business Media; Volume: 116; Issue: 11 Linguagem: Inglês

10.1007/s10482-023-01877-2

1572-9699

Ofélia Godinho, Dominika Klimek, Adrianna Jackiewicz, Bárbara Guedes, Eduarda Almeida, Rita Calisto, Inês Rosado Vitorino, José Diogo Neves dos Santos, Ignacio González, Alexandre Lobo‐da‐Cunha, Magdalena Całusińska, Sandra Quinteira, Olga Maria Lage,

Actinomycetales infections and treatment

Abstract A bacterial strain was isolated from a brackish water sample of Tagus river, Alcochete, Portugal and was designated TO1_6 T . It forms light pink colonies on M13 medium supplemented with N -acetylglucosamine. Cells are pear-shaped to spherical, form rosettes and divide by budding. Strain TO1_6 T presents a mesophilic and neutrophilic profile, with optimum growth at 20 to 25 °C and pH 7.0 to 7.5, and vitamin supplementation is not required to promote its growth. The genome of the novel isolate is 7.77 Mbp in size and has a DNA G + C content of 56.3%. Based on its 16S rRNA gene sequence, this strain is affiliated with the phylum Planctomycetota . Further taxonomic characterization using additional phylogenetic markers, namely rpoB gene sequence (encoding the β-subunit of the DNA-dependent RNA polymerase), as well as Percentage of conserved proteins, average nucleotide identity and average amino acid identity, suggest the affiliation of strain TO1_6 T to the genus Stieleria , a recently described taxon in the family Pirellulaceae, order Pirellulales and class Planctomycetia . Based on the genotypic, phylogenetic and physiological characterization, we here describe a new species represented by the type strain TO1_6 T (= CECT 30432 T , = LMG 32465 T ), for which the name Stieleria tagensis sp. nov. is proposed.