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Effect of intramammary infection and inflammation on milk protein profile assessed at the quarter level in Holstein cows

2023; Elsevier BV; Volume: 107; Issue: 3 Linguagem: Inglês

10.3168/jds.2023-23818

1529-9066

Vittoria Bisutti, A. Vanzin, Sara Pegolo, Alessandro Toscano, Matteo Gianesella, Enrico Sturaro, Stefano Schiavon, Luigi Gallo, Franco Tagliapietra, Diana Giannuzzi, Alessio Cecchinato,

Probiotics and Fermented Foods

In this study we wanted to investigate the associations between naturally occurring subclinical intramammary infection (IMI) caused by different etiological agents (i.e., Staphylococcus aureus, Streptococcus agalactiae, Streptococcus uberis, and Prototheca spp.), in combination with somatic cell count (SCC), on the detailed milk protein profile measured at the individual mammary gland quarter. An initial bacteriological screening (time 0; T0) conducted on individual composite milk from 450 Holstein cows reared in 3 herds, was performed to identify cows with subclinical IMI. We identified 78 infected animals which were followed up at the quarter level at 2 different sampling times: T1 and T2, 2 and 6 wk after T0, respectively. A total of 529 quarter samples belonging to the previously selected animals were collected at the 2 sampling points and analyzed with a reversed phase HPLC (RP-HPLC) validated method. Specifically, we identified and quantified 4 caseins (CN), namely α