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Disposable and eco-friendly electrochemical immunosensor for rapid detection of SARS-CoV-2

2023; Elsevier BV; Volume: 268; Linguagem: Inglês

10.1016/j.talanta.2023.125337

1873-3573

Danielly S. Rocha, Thaísa A. Baldo, Habdias A. Silva‐Neto, Gerson F. Duarte‐Junior, Gabriela Silvério Bazílio, Clayton Luiz Borges, Juliana Alves Parente-Rocha, William R. de Araújo, Abner de Siervo, Thiago R. L. C. Paixão, Wendell K. T. Coltro,

Biosensors and Analytical Detection

This study describes the development of a simple, disposable, and eco-friendly electrochemical immunosensor for rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Electrochemical devices were manufactured by stencil-printing using low-cost materials such as polyester sheets, graphite flakes, and natural resin. The immunosensor comprises gold nanoparticles stabilized with cysteamine, glutaraldehyde, anti-SARS-CoV-2 S protein monoclonal antibody (Ab1) as the biological receptor, and bovine serum albumin as a protective layer. The COVID-19 diagnostic was based on rapid square wave voltammetry measurements (15 min) using [Fe(CN)6]3-/4- as a redox probe. The method presented a linear response in the concentration range from 250 pg mL−1 to 20 μg mL−1 S protein, with a limit of detection of 36.3 pg mL−1. The proposed immunosensor was stable for up to two weeks when stored at 4 °C and it demonstrated excellent clinical performance in diagnosing COVID-19 when applied to a panel of 44 undiluted swab samples collected from symptomatic patients. In comparison with results obtained through the quantitative reverse transcription polymerase chain reaction method, the proposed immunosensor offered 100 % accuracy, thus emerging as a powerful alternative candidate for routine and decentralized testing, which can be helpful in controlling the COVID-19 outbreak.