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Application Studies of Alkaline Protease from Marine Engyodontium Album BTMF S10 in Detergent Industry and in Silver Recovery from Used X-Ray Films

2023; Volume: 59; Issue: 3 Linguagem: Inglês

10.36460/kavaka/59/3/2023/26-35

0379-5179

Sreeja Chellapan,

Corrosion Behavior and Inhibition

Alkaline protease isolated from marine fungus Engyodontium album BTMFS10 was evaluated for its potential use as an additive in detergent formulations in detergent industry.Commercial detergent compatibility studies conducted with the E. album protease indicated that in all the detergents tested, the enzyme retained more than 90% of activity even after 3 h of incubation.Comparison of stability of E. album protease and other proteases in the presence of commercial detergent (Surf Excel Automatic) at 60 °C showed that E. album protease was significantly more active than the commercially available proteases tested.Additionally, wash performance research supported the efficiency of E. album protease in removing blood stains from clothing.Esterase activity of the protease indicated by the cleavage of p-nitrophenyl derivatives of small chain fatty acids and was confirmed by activity staining.This fungal protease facilitated stripping of the gelatin layer of the used X-ray film towards recovery of silver.The results of the studies testified the potential of marine fungal protease for application as an addition in detergent compositions, and also in silver recovery from used X-ray films.