Produção Nacional
Revisado por pares
A voltammetric peptide biosensor for Cu2+ metal ion quantification in coffee seeds
2023; Elsevier BV; Volume: 251; Linguagem: Inglês
10.1016/j.jinorgbio.2023.112441
ISSN1873-3344
AutoresGabriella Magarelli, Jonatas Gomes da Silva, Caroline Luchtenberg Ribeiro, Thiago Viana de Freitas, Magali Aparecida Rodrigues, Eric de Souza Gil, Pierre Marraccini, Jurandir Rodrigues de Souza, Clarissa Silva Pires de Castro, Marcelo P. Bemquerer,
Tópico(s)Electrochemical Analysis and Applications
ResumoA prion-derived copper(II)-binding peptide was assembled onto a gold electrode for the building of a voltammetric biosensor for measuring the Cu2+ metal ion in biological samples. The chosen sequence was H-CVNITKQHTVTTTT-NH2, with an appended cysteine residue for binding to the gold surface as a self-assembled monolayer and a histidine residue as the anchorage point for copper(II) complexation. The biosensor showed a linear range of 10−7 to 10−6 M with an 8.0 × 10−8 M detection limit and a 1.0 × 10−7 M quantification limit, with good precision, trueness, and absence of matrix effect. The quantification of Cu2+ was performed in the presence of other transition metal ions, such as Zn2+, Cd2+, Fe2+, or Ni2+, which indicates the excellent selectivity of the biosensor. When the modified electrode was applied for measuring copper(II) in calcined coffee seeds, a difference in copper amount was observed between two Coffea arabica cultivars that were submitted to a treatment with a copper-based antifungal, showing the applicability of the biosensor in the agricultural field.
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