Portal de Periódicos da CAPES

Artigo Produção Nacional Revisado por pares

BIBTEX RIS

SARS-CoV-2 Nanovaccine Composed of Microfluidic-Produced Gold Nanoparticles Induces Neutralizing Immune Responses

2023; American Chemical Society; Volume: 6; Issue: 24 Linguagem: Inglês

10.1021/acsanm.3c03905

2574-0970

Natália Cristina Dalibera, Mônica Josiane Rodrigues‐Jesus, Robert Andreata‐Santos, Luiz Mário Ramos Janini, Aline Furtado Oliveira, Adriano Rodrigues Azzoni, Luís Carlos de Souza Ferreira, Marianna Teixeira de Pinho Favaro,

SARS-CoV-2 detection and testing

The pursuit of effective and safe vaccines against severe acute respiratory syndrome caused by SARS-CoV-2 has posed a significant challenge for science in recent years, allowing the emergence of new vaccine strategies and platforms. Here, we present a coronavirus disease candidate vaccine based on gold nanoparticles (AuNPs), synthesized using a microfluidic process and conjugated to a SARS-CoV-2 Spike protein receptor binding domain (RBD). AuNPs of different sizes and morphologies were able to bind the RBD protein. Immunization of C57BL/6 mice demonstrated that conjugated AuNPs have consistently improved antigen-specific antibody responses in comparison with RBD alone, not only inducing higher IgG titers but also boosting the neutralizing capacity of antibodies. In addition, immunization with AuNPs of different sizes and morphologies led to significant differences in serum-virus-neutralizing antibody titers, suggesting that these factors play an essential role in the interaction of AuNPs and the immune system. In conclusion, our study highlights the use of conjugated AuNPs as an antigen-delivery system for protein-based vaccines against SARS-CoV-2 and opens perspectives for the application of this technological platform for the future development of nanoparticle-based vaccines against other infectious diseases.