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Laser scanning cytometry: The future of conventional flow cytometry is past

2023; Taylor & Francis; Volume: 52; Issue: 5 Linguagem: Inglês

10.1080/10739149.2023.2292269

1525-6030

Gabrielly Pereira Ribeiro, Dominik Lenz,

Image Processing Techniques and Applications

Flow cytometry (FC) was developed in the late 1950s and is nowadays the gold standard for automated quantitative cellular analysis. In the early 1990s, a new generation of cytometry became commercially available—laser scanning cytometry (LSC). While the hardware of LSC was related to FC, LSC provided images of forward scatter and all fluorochromes. This new technique allowed the analysis of solid tissues and reanalysis of samples and provided data similar to those of FC. A number of peer-reviewed, frequently cited studies were published using the features of LSC. Only a minority, however, must have believed that LSC would replace FC by that time. Currently, the LSC is still sold but withheld from any further development. It seems an epoque has come to an end. How did it come? The presented literature review tries to answer some questions.