Produção Nacional
Revisado por pares
IAPT chromosome data 40
2023; Wiley; Volume: 72; Issue: 6 Linguagem: Inglês
10.1002/tax.13102
ISSN1996-8175
AutoresKarol Marhold, Jaromír Kučera, Fernanda Pessi de Abreu, Diego Alarcón, Erton Mendonça de Almeida, William Santana Alves, Maria de Fátima de Araújo Lucena, Carlos M. Baeza, Isabela Andrade Bahima, Ana Flávia Santos de Brito, Érica Pereira de Campos, Antônio Gabriel Torres Cardoso, Daniela Santos Carneiro‐Torres, Luana Crestani de Carvalho, Marcus Alberto Nadruz Coelho, Joel Maciel Pereira Cordeiro, Pedro Costales‐Maestre, Julia Gabriele Dani, Zoila Díaz Lifante, Jaime Espejo, Leonardo P. Félix, Joana Focchezatto, Pablo García‐Moro, Guilherme Pecoits Goldstein, Felipe Gonzatti, Arneet Grewal, Catherine Guzati, Pedro Jiménez‐Mejías, Eliane Kaltchuk‐Santos, H. Kaur, Satinder Kaur, Ilia J. Leitch, Talita Kelly Pinheiro Lucena, Farhana Majid, José Ignacio Márquez‐Corro, Santiago Martín‐Bravo, José A. Mejías, Adryene Mota de Menezes, Sahr Mian, Paulo Muñoz‐Schüler, Rodrigo Garcia Silva Nascimento, Sarah do Nascimento, Felipe Nollet, Patricio Novoa, Regina Célia de Oliveira, Jaume Pellicer, Diego Penneckamp, Diego Sotero de Barros Pinangé, Poonam Rani, Priscila A. Reis, André Rodolfo de Oliveira Ribeiro, Gloria Rojas, Amanda Santos, Angeline Maria da Silva Santos, María Sanz‐Arnal, Giulia Melilli Serbin, Anádria Stéphanie da Silva, Rosemere dos Santos Silva, Andressa Dantas da Silveira, Graciele Lurdes Silveira, Gustavo Souza, Tatiana T. Souza‐Chies, Eudes Maria Stiehl‐Alves, Luana Olinda Tacuatiá, Lu Tan, Sebastián Teillier, Eitel Thielemann, Caroline Trevelin, Ariane Tonetto Vieira, Larissa Fonseca Andrade‐Vieira,
Tópico(s)CRISPR and Genetic Engineering
ResumoKM, https://orcid.org/0000-0002-7658-0844; JK, https://orcid.org/0000-0002-9983-7630; FPA, https://orcid.org/0000-0002-5006-4833; DA, https://orcid.org/0000-0003-3751-047X; EMA, https://orcid.org/0000-0001-8033-5334; WSA, https://orcid.org/0000-0001-7443-6008; MFA, https://orcid.org/0000-0002-9612-6614; CMB, https://orcid.org/0000-0001-9546-5721; IAB, https://orcid.org/0009-0006-7941-0417; AFSB, https://orcid.org/0000-0002-5641-024X; EPC, https://orcid.org/0000-0001-6972-3555; AGTC, https://orcid.org/0000-0002-8948-7817; DSC-T, https://orcid.org/0000-0002-4561-8811; LCC, https://orcid.org/0000-0002-2105-9859; MANC, https://orcid.org/0000-0003-1039-6777; JMPC, https://orcid.org/0000-0001-9833-0822; PC-M, https://orcid.org/0009-0008-4154-0368; JGD, https://orcid.org/0000-0002-2724-9346; ZDL, https://orcid.org/0000-0002-5277-9082; JE, https://orcid.org/0000-0002-4999-1883; LPF, https://orcid.org/0000-0001-9202-9828; JF, https://orcid.org/0009-0005-4241-3123; PGM, https://orcid.org/0000-0002-8331-4771; GPG, https://orcid.org/0009-0009-8691-5714; FG, https://orcid.org/0000-0003-1971-0558; AG, https://orcid.org/0000-0001-5355-9666; CG, https://orcid.org/0000-0002-3414-7989; PJ-M, https://orcid.org/0000-0003-2815-4477; EK-S, https://orcid.org/0000-0002-0002-0234; HK, https://orcid.org/0009-0002-5724-2695; SK, https://orcid.org/0000-0002-8919-1856; IJL, https://orcid.org/0000-0002-3837-8186; TKPL, https://orcid.org/0000-0002-8830-8450; FM, https://orcid.org/0009-0006-4772-0576; JIM-C, https://orcid.org/0000-0003-4277-2933; SM-B, https://orcid.org/0000-0003-0626-0770; JAM, https://orcid.org/0000-0001-6393-6496; AMM, https://orcid.org/0009-0001-7985-103X; SM, https://orcid.org/0000-0001-7828-0629; PM-S, https://orcid.org/0009-0001-7070-4121; RGSN, https://orcid.org/0000-0002-7133-4994; SN, https://orcid.org/0009-0001-7200-7493; FN, https://orcid.org/0000-0002-1362-685X; PN, https://orcid.org/0000-0003-2108-9449; RCO, https://orcid.org/0000-0001-7891-2630; JP, https://orcid.org/0000-0001-7632-9775; DNP, https://orcid.org/0000-0002-0760-4128; DSBP, https://orcid.org/0000-0001-8559-7894; PR, https://orcid.org/0000-0003-0698-2757; PAR, https://orcid.org/0009-0004-4982-0269; AROR, https://orcid.org/0000-0002-0085-3113; GR, https://orcid.org/0000-0003-2167-6774; ASS, https://orcid.org/0000-0003-1061-9504; AMSS, https://orcid.org/0000-0001-8765-8291; MS-A, https://orcid.org/0000-0003-0651-243X; GMS, https://orcid.org/0000-0003-4266-4422; ASS, https://orcid.org/0000-0001-8023-4963; RSS, https://orcid.org/0000-0003-1838-6362; ADS, https://orcid.org/0000-0002-9177-9384; GLS, https://orcid.org/0000-0003-1728-4006; GS, https://orcid.org/0000-0002-5700-6097; TTS-C, https://orcid.org/0000-0002-1818-8748; EMS-A, https://orcid.org/0000-0003-1733-7315; LOT, https://orcid.org/0009-0003-0530-7888; LT, https://orcid.org/0000-0002-4235-5382; ST, https://orcid.org/0000-0002-3785-0434; ET, https://orcid.org/0009-0001-3779-214X; CT, https://orcid.org/0000-0002-0562-7027; ATV, https://orcid.org/0000-0003-3366-5001; LFAV, https://orcid.org/0000-0002-7947-7498 *Address for correspondence: [email protected] This study was supported by CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil – grants no. 130071/2022-6 and 131999/2021-4), CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil – grant no. 88887.648978/2021-00) and FAPERGS (Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Brazil – grant no. 21/2551-0000780-0). We acknowledge Universidade Federal do Rio Grande do Sul and Universidade de Caxias do Sul for the support provided to this research. We acknowledge laboratory technician Pedro Erê Disconzi Brum for his help in handling the cytometer to obtain genome size data for this work. We would also like to thank colleagues Fernando Fernandes de Oliveira Neto, Gustavo Pedroso de Moraes, Guilherme Pecoits Goldstein and Taciane Schroder Jorge for the support and assistance in the analyses performed. All materials CHN; all records from Brazil; all vouchers in HUCS. Hymenophyllum asplenioides (Sw.) Sw., n = 36; Rio Grande do Sul, F. Gonzatti & F.P. Abreu 6844. Hymenophyllum fucoides (Sw.) Sw., n = 56; Paraná, F. Gonzatti, F.P. Abreu & I.A. Bahima 7244. Hymenophyllum magellanicum (Desv.) Willd. ex Kunze, n = 27; Paraná, F. Gonzatti, F.P. Abreu & I.A. Bahima 7250; Rio Grande do Sul, F. Gonzatti & F.P. Abreu 6845; Santa Catarina, F. Gonzatti, F.P. Abreu & P.L. Casa 6996. Hymenophyllum megachilum C.Presl, n = 13; Rio Grande do Sul, F. Gonzatti & F.P. Abreu 6846; Santa Catarina, F. Gonzatti & F.P. Abreu 6992. *Address for correspondence: [email protected] This study was funded by projects Flora of Chile, Flora of Biobio Region, University of Concepcion, Chile, and Institute of Ecology and Biodiversity (IEB), ANID/BASAL FB210006, Chile. All materials CHN. Alstroemeria piperata A.R.Flores & J.M.Watson, 2n = 16; Chile, Valparaiso Region, G. Rojas 2590 (SGO). Alstroemeria pulchra var. maxima Phil., 2n = 16; Chile, Valparaiso Region, N. Villalobos 1911 (CONC). Miersia chilensis Lindl., 2n = 22; Chile, O'Higgins Region, I. Escobar 74 (CONC). Phycella australis Ravenna, 2n = 16; Chile, Biobio Region, C. Baeza & E. Thielemann 4436 (CONC). Rhodolirium montanum Phil., 2n = 16; Argentina, Córdoba Province, 24 Feb 2023, J. Espejo s.n. (CONC). Zephyranthes maculata (L'Hér.) Nic.García, 2n = 18; Chile, Biobio Region, C. Baeza 4439 (CONC). Zephyranthes monantha (Ravenna) Nic.García, 2n = 18; Chile, Biobio Region, C. Baeza 4435 (CONC). Zephyranthes aff. splendens (Renjifo) Nic.García, 2n = 18; Chile, Biobio Region, C. Baeza 4447a (CONC). Zephyranthes splendens (Renjifo) Nic.García, 2n = 18; Chile, Biobio Region, C. Baeza 4435 (CONC). Agoseris coronopifolia (d'Urv.) K.L.Chambers, 2n = 18; Chile, Biobio Region, C. Baeza 4463 (CONC); Chile, Metropolitana Region, S. Teillier & al. 8626 (CONC). Chaetanthera microphylla (Cass.) Hook. & Arn., 2n = 24; Chile, Biobio Region, C. Baeza 4439a (CONC). Chaetanthera moenchioides Less., 2n = 26; Chile, Biobio Region, C. Baeza 4447 (CONC). Crepis capillaris (L.) Wallr., 2n = 6; Chile, Biobio Region, C. Baeza 4459 (CONC). Crepis pulchra L., 2n = 8; Chile, Biobio Region, S. Teillier & al. 8642 (CONC). Haplopappus bustillosianus J.Rémy, 2n = 10; Chile, Biobio Region, S. Teillier & al. 8643 (CONC). Haplopappus glutinosus Cass., 2n = 10; Chile, Biobio Region, S. Teillier & al. 8644 (CONC). Haplopappus paucidentatus Phil., 2n = 10; Chile, Biobio Region, S. Teillier & al. 8645 (CONC). Hieracium glaucifolium Poepp. ex Froel., 2n = 18; Chile, Biobio Region, C. Baeza 4455a (CONC). Hypochaeris acaulis (J.Rémy) Britton, 2n = 8; 2n = 16; Chile, Biobio Region, C. Baeza 4464a (CONC). Hypochaeris apargioides Hook. & Arn., 2n = 8; Chile, Biobio Region, C. Baeza 4444a, 4459a (CONC). Hypochaeris clarionoides (J.Rémy) Reiche, 2n = 8; Chile, Metropolitana Region, 15 Jan 2023, J. Espejo s.n. (CONC). Hypochaeris gayana (DC.) Cabrera, 2n = 8; Chile, Biobio Region, C. Baeza 4458 (CONC). Hypochaeris glabra L., 2n = 10; Chile, Biobio Region, C. Baeza 4444b (CONC). Hypochaeris radicata L., 2n = 8; Chile, Biobio Region, C. Baeza & al. 4469 (CONC). Hypochaeris scorzonerae (DC.) F.Muell., 2n = 8; Chile, Valparaíso Region, 22 Mar 2023, P. Novoa s.n. (CONC). Lactuca serriola L., 2n = 18; Chile, Biobio Region, C. Baeza 4455 (CONC). Leontodon hirtus L., 2n = 8; Chile, Valparaiso Region, 18 Apr 2022, P. Novoa s.n. (CONC). Leontodon saxatilis Lam., 2n = 8; Chile, Biobio Region, 15 Jan 2021, C. Baeza s.n. (CONC). Noticastrum antucense Phil., 2n = 8; Chile, Biobio Region, C. Baeza 4465 (CONC). Notopappus prunelloides (Poepp. ex Less.) Klingenb., 2n = 12; Chile, Biobio Region, C. Baeza 4460 (CONC). Picris echioides L., 2n = 10; Chile, Biobio Region, C. Baeza 4457 (CONC). Lathyrus magellanicus Lam., 2n = 14; Chile, Biobio Region, S. Teillier & al. 8646 (CONC). Azara serrata Ruiz & Pav. var. serrata, 2n = 18; Chile, Biobio Region, 20 Jan 2022, C. Baeza s.n. (CONC). *Address for correspondence: [email protected] This study was supported by research projects CGL2010-16512 and PGC2018-099608-B-100 (REPROGRAD) of the Spanish Ministry of Science and Innovation. All materials CHN. Narcissus cuatrecasasii Fern.Casas, M.Laínz & Ruiz Rejón, 2n = 14; Spain, Mejías s.n. (SEV 289807). Muscari armeniacum Leichtlin ex Baker, 2n = 18; Turkey, Mejías & Sánchez s.n. (SEV 256836); Turkey, Mejías & Sánchez s.n. (SEV 256837). Sonchus fragilis Ball, 2n = 18; Morocco, Ajbilou, Arroyo, Marañón & Mejías s.n. (SEV 215728, SEV 217382). Sonchus masguindalii Pau & Font Quer, 2n = 18, n = 9; Morocco, Arroyo & Mejías s.n. (SEV 215733, SEV 217381). Sonchus nymanii Tineo & Guss., 2n = 18; Italy, Sicily, Lim, Mejías & Silva IT27/11 (SEV 284705), Lim, Mejías & Silva IT22/11 (SEV 284703). Sonchus pustulatus Willk., 2n = 18; Morocco, Mejías s.n. (SEV 217476, SEV 217476). *Address for correspondence: [email protected] The study was supported by financial grant under JRF programme of CSIR providing Junior Research Fellowship financial assistance to Poonam Rani under scheme of CSIR (award letter no. 09/140(0161)/2016-EMR-I). All materials CHN; collectors: FM = Farhana Majid, HK = Hardeesh Kaur, PR = Poonam Rani, SK = Satinder Kaur. Mammillaria carnea Zucc. ex Pfeiff., n = 11; India, Haryana, Panchkula, HK s.n. (PUN 39015). Mammillaria magnimamma Haw., n = 11; India, Haryana, Panchkula, HK s.n. (PUN 39065); India, Punjab, Jalandhar, HK s.n. (PUN 39066). Murdannia nudiflora (L.) Brenan., n = 10 + 0–1B; India, Himachal Pradesh, PR s.n. (PUN 63192, PUN 63194, PUN 63195). Tradescantia pallida (Rose) D.R.Hunt., n = 12 + 0–1B; India, Himachal Pradesh, PR s.n. (PUN 63468). Costus pictus D.Don., n = 14; India, Punjab, PR s.n. (PUN 63178). Mentha arvensis L., n = 12; India, Jammu and Kashmir, FM s.n. (PUN 62365). Scutellaria galericulata L., n = 16; India, Jammu and Kashmir, FM s.n. (PUN 62395). Stachys sericea Wall., n = 16; India, Jammu and Kashmir, FM s.n. (PUN 62405). Sida acuta Burm.f., n = 14 + 0–1B; India, Himachal Pradesh, SK s.n. (PUN 62698, PUN 62699). *Address for correspondence: [email protected] This research was supported by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil – grant number 88887.648978/2021-00), CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil – grant number 130071/2022-6 and 131999/2021-4) and FAPERGS (Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Brazil – grant number 21/2551-0000780-0). We acknowledge laboratory technician Pedro Erê Disconzi Brum for his help in handling of the cytometer to obtain genome size data of this work. All materials CHN. Subfamily Iridoideae Cypella altouruguaya Chauveau & L.Eggers, 2n = 14; Brazil, Rio Grande do Sul, L. Eggers & O. Chauveau 716 (ICN). Cypella amplimaculata Chauveau & L.Eggers, 2n = 14; Brazil, Rio Grande do Sul, J.G. Dani & C. Guzati 280 (ICN); Brazil, Rio Grande do Sul, J.G. Dani, C. Guzati & H.M. Buneker 375 (ICN); Brazil, Santa Catarina, J.G. Dani, C. Guzati & H.M. Buneker 330 (ICN). Cypella discolor Chauveau & L.Eggers, 2n = 4x = 28; Brazil, Rio Grande do Sul, E.M. Stiehl-Alves, F.S. Silveira, J. Focchezatto & L.O. Tacuatiá 94 (ICN). Cypella fucata Ravenna, 2n = 14; Brazil, Rio Grande do Sul, J.G.Dani, C. Guzati, L.N. Da Silva & G. Pecoits 318 (ICN); Brazil, Rio Grande do Sul, J.G. Dani, C. Guzati & H.M. Buneker 374 (ICN); Brazil, Rio Grande do Sul, J.G. Dani & C. Guzati 386 (ICN). Cypella hauthalii subsp. minuticristata Chauveau & L.Eggers, 2n = 14; Brazil, Rio Grande do Sul, J.G. Dani, C. Guzati & H.M. Buneker 376 (ICN). Cypella herbertii subsp. herbertii Herb., 2n = 14; Brazil, Santa Catarina, J.G. Dani, C. Guzati & H.M. Buneker 364 (ICN). Cypella luteogibbosa Deble, 2n = 14; Brazil, Rio Grande do Sul, E.M. Stiehl-Alves, F.S. Silveira, J. Focchezatto & L.O. Tacuatiá 96 (ICN). Cypella magnicristata Deble, 2n = 14; Brazil, Rio Grande do Sul, E.M. Stiehl-Alves, F.S. Silveira, J. Focchezatto & L.O. Tacuatiá 98 (ICN). Cypella pusilla (Link & Otto) Benth. & Hook.f. ex B.D.Jacks., 2n = 14; Brazil, Rio Grande do Sul, T. Pastori & O. Chauveau 191 (ICN); Brazil, Rio Grande do Sul, T. Pastori, M. Chiara-Moço, P.J.S. Silva-Filho, R. Macedo & L. Oliveira 148 (ICN); Brazil, Rio Grande do Sul, T. Pastori, M. Longhi, C. Forgiarini & L. Nogueira 168 (ICN); Brazil, Rio Grande do Sul, Caçapava do Sul, L. Eggers & O. Chauveau 914 (ICN). Gelasine aff. coerulea (Vell.) Ravenna, 2n = 14; Brazil, Santa Catarina, 5 Oct 2017, C. Valus & A. Silvério s.n. (MBM). Gelasine elongata (Graham) Ravenna, 2n = 12; Brazil, Rio Grande do Sul, L. Eggers & T.T. Souza-Chies 254 (ICN). Gelasine uruguaiensis Ravenna, 2n = 14; Brazil, Rio Grande do Sul, L.P. Deble & A.S. de Oliveira-Deble 13578 (ICN). *Address for correspondence: [email protected] Financial support from CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior). All materials CHN; collectors: EMA = E.M. Almeida, EMN = E.M. Neto, LPF = L.P. Felix, SN = S. Nascimento; vouchers in EAN (Herbarium Prof. Jayme Coelho de Moraes). Anthurium andraeanum Linden ex André, 2n = 36; Brazil, Paraiba, LPF 19523. Anthurium bromelicola Mayo & L.P.Felix, 2n = 30; Brazil, Paraiba, LPF 19524. Anthurium harleyi T.A.Pontes & Mayo, 2n = 30 + 1B; Brazil, Bahia, LPF 18315. Anthurium ianthinopodum (Schott ex Engl.) Nadruz & Mayo, 2n = 30 + 2B; Brazil, Espírito Santo, EMA 1329. Anthurium minarum Sakur. & Mayo, 2n = 32; Brazil, Minas Gerais, LPF 19200. Anthurium pentaphyllum (Aubl.) G.Don, 2n = 30; Brazil, São Paulo, LPF 16970. Anthurium petrophilum K.Krause, 2n = 30; Brazil, Bahia, LPF 18374. 2n = 30 + 1B; Brazil, Bahia, E.M. Almeida 966; Brazil, Paraiba, SN 150; Brazil, Pernambuco, EMN 49. 2n = 30 + 2B; Brazil, Bahia, EMA 616. Anthurium raimundii Mayo, Haigh & Nadruz, 2n = 30 + 1B; Brazil, Pernambuco, LPF 17818. Anthurium scandens (Aubl.) Engl., 2n = 48; Brazil, Bahia, LPF 18313; Brazil, Bahia, EMA 1028; Brazil, Pernambuco, LPF 18667; Brazil, Pernambuco, LPF 18678. Anthurium sellowianum Kunth, 2n = 60; Brazil, São Paulo, LPF 16810. Anthurium urvilleanum Schott, 2n = 30 + 8B; Brazil, São Paulo, LPF 16998. Philodendron acutatum Schott 2n = 32; Brazil, Piauí, EMA 1099. 2n = 32 + 1B; Brazil, São Paulo, LPF 17003. Syngonium podophyllum Schott, 2n = 24; Brazil, Paraiba, LPF 13663. *Address for correspondence: [email protected] This study was supported by the “IAPT Research Grant 2022” awarded to J.I. Márquez-Corro, who also was granted by the “Next Generation EU” funding, the Recovery, Transformation and Resilience Plan and the Ministry of Universities, under the grants “Margarita Salas” for the requalification of the Spanish university system 2021–2023 called by the Universidad Pablo de Olavide, Seville. The work was partly funded by a project granted to P. Jiménez-Mejías and S. Martín-Bravo by the Spanish Ministry of Science and Innovation (PID2020-113897GB-I00). The authors thank A. Marticorena, P. López Sepúlveda, G. Fuentes Arce, and C.M. Baeza for technical support with the herbarium material and other aspects of J.I. Márquez-Corro's stay at Universidad de Concepción (Chile). Moreover, the authors really appreciate S. Molino's help with the identification of some fern specimens. Finally, a special mention to B. Peceño Capilla and J.P. Cortes Pastén for logistic support during the first part of the fieldwork. The authors would also like to thank the Government of Chile (CONAF, Ministry of Agriculture) for granting permission to collect the plant material (Permit authorization number 048/2022). Fentxen chaltumay. All materials FCM; collectors: JIM-C = J.I. Márquez-Corro, MS-A = M. Sanz-Arnal, PJ-M = P. Jiménez-Mejías, PM-S = P. Muñoz-Schüler, SM-B = S. Martín-Bravo. Aextoxicon punctatum Ruiz & Pav., 2C = 2.23 ± 0.022 pg, CV = 3.60; Chile, Biobío, JIM-C & PM-S 87JMC23 (UPOS). Austroblechnum lechleri (T.Moore) Gasper & V.A.O.Dittrich, 2C = 23.65 ± 0.121 pg, CV = 3.22; Chile, Biobío, PM-S & al. PMS-243 (CONC, MACB). Blechnum sp., 2C = 17.75 ± 0.069 pg, CV = 4.03; Chile, Araucanía, SM-B & al. 46bisSMB23 (CONC, MACB). Parablechnum chilense (Kaulf.) Gasper & Salino, 2C = 27.03 ± 0.188 pg, CV = 4.48; Chile, Ñuble, SM-B & al. 15SMB23 (CONC, MACB). 2C = 26.64 ± 0.084 pg, CV = 4.03; Chile, Los Lagos, PJ-M & al. 31PJM-CL23 (CONC, MACB). Puya alpestris (Poepp.) Gay, 2C = 1.00 ± 0.001 pg, CV = 3.99; Chile, Coquimbo, JIM-C & PM-S 82JMC23 (UPOS). Maytenus boaria Molina, 2C = 2.84 ± 0.008 pg, CV = 3.22; Chile, Biobío, JIM-C & PM-S 88JMC23 (UPOS). Weinmannia trichosperma Cav., 2C = 0.98 ± 0.031 pg, CV = 7.75; Chile, Biobío, Concepción, JIM-C & PM-S 93JMC23 (UPOS). Fitzroya cupressoides (Molina) I.M.Johnst., 2C = 35.28 ± 0.141 pg, CV = 4.11; Chile, Los Ríos, JIM-C & PM-S 83JMC23 (CONC). Dioscorea brachybotrya Poepp., 2C = 1.89 ± 0.009 pg, CV = 3.77; Chile, Biobío, PM-S & al. PMS-241 (CONC, UPOS). Avellanita bustillosii Phil., 2C = 0.52 ± 0.005 pg, CV = 4.21; Chile, Metropolitana, MS-A & al. 27MSA-CL23 (CONC, MA). Astragalus cruckshanksii (Hook. & Arn.) Griseb., 2C = 2.26 ± 0.019 pg, CV = 2.67; Chile, Coquimbo, PM-S & al. PMS-197 (CONC). Sophora cassioides (Phil.) Sparre, 2C = 1.80 ± 0.038 pg, CV = 3.83; Chile, Biobío, Concepción, JIM-C & PM-S 86JMC23 (UPOS). Gomortega keule (Molina) Baill., 2C = 2.93 ± 0.009 pg, CV = 3.16; Chile, Biobío, JIM-C & al. 79bisJMC23 (UPOS). Juncus microcephalus Kunth, 2C = 1.09 ± 0.003 pg, CV = 3.12; Chile, Coquimbo, PM-S & al. PMS-222 (CONC, UPOS). Juncus stipulatus Nees & Meyen, 2C = 0.93 ± 0.015 pg, CV = 4.22; Chile, Ñuble, SM-B & al. 09SMB23 (CONC, UPOS). Patosia clandestina (Phil.) Buchenau, 2C = 1.37 ± 0.005 pg, CV = 2.60; Chile, Coquimbo, PM-S & al. PMS-209 (CONC, UPOS). Cryptocarya alba (Molina) Looser, 2C = 2.34 ± 0.021 pg, CV = 3.52; Chile, Biobío, JIM-C & PM-S 84JMC23 (UPOS). Peumus boldus Molina, 2C = 5.18 ± 0.079 pg, CV = 2.87; Chile, Biobío, JIM-C & PM-S 89JMC23 (UPOS). Luma apiculata (DC.) Burret, 2C = 1.02 ± 0.017 pg, CV = 4.27; Chile, Biobío, Concepción, JIM-C & PM-S 90JMC23 (UPOS). Nothofagus obliqua (Mirb.) Oerst., 2C = 1.19 ± 0.001 pg, CV = 3.52; Chile, Biobío, JIM-C & PM-S 91JMC23 (UPOS). Podocarpus salignus D.Don, 2C = 17.78 ± 0.332 pg, CV = 4.73; Chile, Biobío, Concepción, JIM-C & PM-S 92JMC23 (UPOS). Apodasmia chilensis (Gay) B.G.Briggs & L.A.S.Johnson, 2C = 2.09 ± 0.014 pg, CV = 4.20; Chile, Los Lagos, PJ-M & al. 32PJM-CL23 (CONC, UPOS). Pitavia punctata (Ruiz & Pav.) Molina, 2C = 1.07 ± 0.006 pg, CV = 3.92; Chile, Biobío, Concepción, JIM-C & PM-S 85JMC23 (UPOS). Drimys winteri J.R.Forst. & G.Forst., 2C = 3.90 ± 0.011 pg, CV = 3.04; Chile, Biobío, JIM-C & PM-S 94JMC23 (UPOS). *Address for correspondence: [email protected] Authors express thanks to CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) for financial support, and INSA (Instituto Nacional do Semiárido) for technical support. All materials CHN; vouchers in EAN (Herbarium Prof. Jayme Coelho de Moraes). Anacardium occidentale L., 2n = 40; Brazil, Pernambuco, L.P. Felix 17303. Astronium fraxinifolium Schott, 2n = 30; Brazil, Paraíba, L.P. Felix 17114. Mangifera indica L., 2n = 40; Brazil, Paraíba, J.M.P. Cordeiro 1337. Myracrodruon urundeuva Allemão, 2n = 30; Brazil, Pernambuco, L.P. Felix 17308. Schinopsis brasiliensis Engl., 2n = 28; Brazil, Paraíba, L.P. Felix 17100. Schinus terebinthifolia Raddi, 2n = 28; Brazil, Paraíba, L.P. Felix 17496. Commiphora leptophloeos (Mart.) J.B.Gillett, 2n = 26; Brazil, Paraíba, L.P. Felix 16687. Celtis iguanaea (Jacq.) Sarg., 2n = 20; Brazil, Paraíba, P.C. Gadelha Neto 4160. Utricularia quelchii N.E.Br., 2n = 18; Brazil, Roraima, L.P. Felix 16222. Amorimia septentrionalis W.R.Anderson, 2n = 20; Brazil, Paraíba, J.M.P. Cordeiro 1339. Azadirachta indica A.Juss., 2n = 28; Brazil, Paraíba, J.M.P. Cordeiro 1342. Cedrela fissilis Vell., 2n = 54; Brazil, Pernambuco, L.P. Felix 15988. Melia azedarach L., 2n = 28; Brazil, Paraíba, J.M.P. Cordeiro 1368. Eugenia zigzag K.Cout. & Sobral, 2n = 22; Brazil, Paraíba, J.M.P. Cordeiro 1544. Caularthron bicornutum Raf., 2n = 40; Brazil, Amazonas, L.P. Felix 16336. Triplaris gardneriana Wedd., 2n = 22; Brazil, Paraíba, L.P. Felix 17561. Randia armata (Sw.) DC., 2n = 40; Brazil, Rio Grande do Norte, R.T. Queiroz 613. Ruta chalepensis L., 2n = 40; Brazil, Paraíba, J.M.P. Cordeiro 1406. Filicium decipiens (Wight & Arn.) Thwaites, 2n = 32; Brazil, Paraíba, J.M.P. Cordeiro 1407. Paullinia pinnata L., 2n = 24; Brazil, Paraíba, L.P. Felix 4777. Sapindus saponaria L., 2n = 28; Brazil, Paraíba, L.P. Felix 13946. Serjania salzmanniana Schltdl., 2n = 24; Brazil, Paraíba, J.M.P. Cordeiro 1333. Talisia esculenta (A.St.-Hil.) Radlk., 2n = 32; Brazil, Paraíba, J.M.P. Cordeiro 1338. Homalolepis bahiensis (Moric.) Devecchi & Pirani, 2n = 32; Brazil, Paraíba, E.M. Almeida 814. Priva bahiensis DC. ex Schauer, 2n = 36; Brazil, Paraíba, J.M.P. Cordeiro 1525. *Address for correspondence: [email protected] Financial support from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior/CAPES, Conselho Nacional de Desenvolvimento Científico e Tecnológico/CNPq (Proc. 310026/2018-0), the last author's research productivity scholarship (Proc. 302213/2019-8), and Fundação de Amparo à Pesquisa do Distrito Federal/FAP-DF (Proc. 0193.000979/2015 and Proc. 0193.002051/2017), Brasília, Brazil. All materials CHN; vouchers in UB. Arthropogon villosus Nees, 2n = 40; Brazil, Distrito Federal, R.C. Oliveira & A.S. Silva 2969; Brazil, Distrito Federal, R.C. Oliveira & A.S. Silva 2971. Mesosetum bifarium (Hack.) Chase, 2n = 16; Brazil, Goiás, A.R.O. Ribeiro 391. Mesosetum cayennense Steud., 2n = 20; Brazil, Goiás, A.R.O. Ribeiro & R.C. Oliveira 362; Brazil, Goiás, P.A. Reis, A.R.O. Ribeiro & J.E.Q. Faria 181. Mesosetum chlorostachyum (Döll) Chase, 2n = 8; Brazil, Amazonas, R.C. Oliveira & al. 3033. Mesosetum elytrochaetum (Hack.) Swallen, 2n = 24; Brazil, Goiás, A.R.O. Ribeiro 377. Mesosetum exaratum (Trin.) Chase, 2n = 26; Brazil, Minas Gerais, A.R.O. Ribeiro & I.A. Conceição 351. Mesosetum gibbosum Renvoize & Filg., 2n = 24; Brazil, Bahia, A.R.O. Ribeiro & J.E.Q. Faria 408. Spheneria kegelii (Müll. Hal.) Pilg., 2n = 20; Brazil, Amazonas, R.C. Oliveira & al. 3007. Tatianyx arnacites (Trin.) Zuloaga & Soderstr., 2n = 60; Brazil, Bahia, A.R.O. Ribeiro & J.E.Q. Faria 411. *Address for correspondence: [email protected] Financial support from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) and INSA (Instituto Nacional do Semiárido). All materials CHN; vouchers in EAN (Herbarium Prof. Jayme Coelho de Moraes). Croton adamantinus Müll.Arg., 2n = 20; Brazil, Paraiba, L.P. Felix 15870. Croton argyrophyllus Kunth, 2n = 20; Brazil, Bahia, L.P. Felix 15575; Brazil, Pernambuco, L.P. Felix 15636. Croton blanchetianus Baill., 2n = 20; Brazil, Paraiba, A.S. Santos 30. Croton campestris A.St.-Hil., 2n = 20; Uruguay, Maldonado, L.P. Felix 16015. Croton floribundus Spreng., 2n = 100; Brasil, Paraiba, L.P. Felix 15874; Brazil, Paraiba, L.P. Felix 15879. Croton glandulosus L., 2n = 16; Brazil, Paraíba, E.M. Almeida 1581. Croton grewioides Baill., 2n = 20; Brazil, Bahia, L.P. Felix 15559; Brazil, Pernambuco, L.P. Felix 15650. Croton heliotropiifolius Kunth, 2n = 20; Brazil, Paraiba, A.S. Santos 02. 2n = 40; Brazil, Ceará, L.P. Felix 14914. Croton hirtus L'Hér., 2n = 16; Brazil, Paraiba, E.M. Almeida 1582. Croton jacobinensis Baill., 2n = 20; Brazil, Paraiba, A.S. Santos 03; Brazil, Paraiba, E.M. Almeida 1579. Croton limae A.P.S.Gomes, M.F.Sales & P.E.Berry, 2n = 100; Brazil, Bahia, L.P. Felix 15554. Croton lombardianus Croizat., 2n = 20; Uruguay, Maldonado, L.P. Felix 16030. Croton pedicellatus Kunth, 2n = 18; Brazil, Paraiba, J.M.P. Cordeiro 1259. Croton pulegiodorus Baill., 2n = 20; Brazil, Bahia, L.P. Felix 15553. 2n = 22; Brazil, Paraiba, A.S. Santos 19. Croton rudolphianus Müll.Arg., 2n = 20; Bazil, Pernambuco, L.P. Felix 15662. Croton sellowii Baill., 2n = 20; Brazil, Paraiba, J.M.P. Cordeiro 600. Croton tricolor Klotzsch ex Baill., 2n = 20; Brasil, Bahia, L.P. Felix 15557. Croton triqueter Lam., 2n = 20; Brazil, Bahia, L.P. Felix 15558. 2n = 40; Brazil, Paraíba, L.P. Felix 15876. Croton urticifolius Lam., 2n = 20; Brasil, Paraíba, A.S. Santos 04. *Address for correspondence: [email protected] The authors are grateful to Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM; Process no. 01.02.016301.03260/2021-03) for financial support; AMM and AFSB also to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; Process no. 131845/2021-7) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES; Process no. 12002011009P8/2021-03) for fellowships. All material CHN. Aldina heterophylla Spruce ex Benth., 2n = 22; Brazil, Amazonas, H.C. de Lima 8201 (NYBG). *Address for correspondence: [email protected] We thank the Universidade Federal de Rondonópolis (UFR) for providing the premises for the collection of samples and the development of the research; the curator and technicians of the UFMT Herbarium for their courteous service and assistance during the visits and consultations of the collection; the Dr. Vânia Helena Techio for contributions to this work; and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for the doctorate scholarship (CAPES scholarship number 001) granted to the first author. All materials CHN; collected in Brazil, Mato Grosso State. Qualea grandiflora Mart., 2n = 22, n = 11; G.L.S. Silveira & al. s.n. (UFMT 44309, UFMT 44308), L.A.S. Nogueira & al. s.n. (UFMT 43790, UFMT 43793, UFMT 43807, UFMT 44307, UFMT 43791). Qualea multiflora Mart., n = 11; L.A.S. Nogueira & al. s.n. (UFMT 43806). Qualea parviflora Mart., 2n = 22, n = 11; L.A.S. Nogueira & al. s.n. (UFMT 43792, UFMT 43789), G.L.S. Silveira & al. s.n. (UFMT 44042). Vochysia cinnamomea Pohl, n = 12; L.A.S. Nogueira & al. s.n. (UFMT 43809, UFMT 43808, UFMT 43805). Vochysia divergens Pohl, 2n = 24; G.L.S. Silveira & al. s.n. (UFMT 44043, UFMT 44044, UFMT 44045). *Address for correspondence: [email protected] This research was partially supported by FUNBIO (Fundo Brasileiro Para A Biodiversidade) number 044/2021, and received funding from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil). It was also supported by PROTAX 2020-Chamada CNPq/MCTI/CONFAP-FAPS, FAPERGS number 151211/2021-3, and CNPq numbers 425650/2018-9 and 441412/2020-3. ATV thanks FUNBIO and CAPES (finance code 001) for the scholarship received. EMSA thanks CAPES/PNPD for scholarships and Neotropical Grassland Conservancy for grants received. LCC thanks CAPES for her scholarship. CT thanks FAPERGS and PROPESQ/UFRGS for her scholarships. We acknowledge the valuable collaboration of Anderson Mello and Igor Hedlund in field collections. We are also thankful to the laboratory technician Letícia Gal for her help in preparation of reagents for cytogenetic analysis. All materials CHN. Collectors: CSAM = A.M. Cristante, E.M. Stiehl-Alves & R.M. Marchioretto; CSVB = A.M. Cristante, E.M. Stiehl-Alves, A. Tonetto-Vieira & R. Becker; ECP = L. Eggers, O. Chauveau & T. Pastori; KSAV = E. Kaltchuk, E.M. Stiehl-Alves & A. Vieira; SAFS = E.M. Stiehl-Alves, C. Forgiarini & F.S. Silveira; SATVCH = E.M. Stiehl-Alves, A. Tonetto-Vieira, L. Carvalho & I. Hedlund; SATVCM = E.M. Stiehl-Alves, A. Tonetto-Vieira, L. Carvalho & A. Mello; TVEC = A. Tonetto-Vieira, L. Eggers & O. Chauveau; TVLNS = A. Tonetto-Vieira & L. Nogueira. Voucher specimens are deposited in ICN. Herbertia darwinii Roitman & J.A.Castillo, 2n = 14; Brazil, Rio Grande do Sul, TVLNS 5. Herbertia aff. lahue (Molina) Goldblatt, 2n = 14; Brazil, Rio Grande do Sul, CSVB 1, CSVB 2, CSAM 12, KSAV 5, KSAV 17, SATVCH 137, SATVCH 145, SATVCH 162, SATVCH 171. Herbertia lahue (Molina) Goldblatt, 2n = 42; Brazil, Rio Grande do Sul, KSAV 6, SATVCM 105, SATVCM 110, SATVCM 117, SATVCH 172. 2n = 56; Brazil, Rio Grande do Sul, KSAV 3, SATVCH 129, SATVCH 134, SATVCH 148, SATVCH 161, SATVCM 104, SATVCM 111, SATVCM 115, SATVCM 123. Herbertia pulchella Sweet, 2n = 28; Brazil, Rio Grande do Sul, SATVCH 133, SATVCH 143, SATVCH 150, SATVCH 165, TVEC 7. Herbertia aff. quareimana Ravenna, 2n = 14; Brazil, Rio Grande do Sul, SAFS 51, SATVCM 106, SATVCM 109. Herbertia quareimana Ravenna, 2n = 28; Brazil, Rio Grande do Sul, ECP 1103, ECP 733A, SATVCM 125, SATVCM 126. Karol Marhold (ed.),1,2 Jaromír Kučera(ed.),1 Fernanda Pessi de Abreu,3 Diego Alarcón,4 Erton M. Almeida,5 William S. Alves,6 Maria de Fátima de Araújo,7 Carlos M. Baeza,8 Isabela Andrade Bahima,9 Ana Flávia Santos de Brito,10 Érica Pereira de Campos,11 Antonio Gabriel Torres Cardoso,12 Daniela S. Carneiro-Torres,13 Luana Crestani Carvalho,3 Marcus A.N. Coelho,14 Joel M.P. Cordeiro,15 Pedro Costales-Maestre,16 Julia Gabriele Dani,17 Zoila Díaz-Lifante,16 Jaime Espejo,8 Leonardo P. Felix,5 Joana Focchezatto,3 Pablo García-Moro,18 Guilherme Pecoits Goldstein,9 Felipe Gonzatti,19 Arneet Grewal,20 Catherine Guzati,17 Pedro Jiménez-Mejías,18 Eliane Kaltchuk-Santos,3 Hardeesh Kaur,20 Satinder Kaur,20 Ilia J. Leitch,21 Talita K.P. Lucena,6 Farhana Majid,20 José Ignacio Márquez-Corro,18,21 Santiago Martín-Bravo,18 José A. Mejías,16 Adryene Mota de Menezes,10 Sahr Mian,21 Paulo Muñoz-Schüler,22 Rodrigo Garcia Silva Nascimento,23 Sarah do Nascimento,5 Felipe Nollet,23 Patricio Novoa,24 Regina Célia de Oliveira,12 Jaume Pellicer,21,25 Diego N. Penneckamp,26 Diego Sotero de Barros Pinangé,27 Poonam Rani,20 Priscila Alves dos Reis,21 André Rodolfo de Oliveira Ribeiro,28 Gloria Rojas,29 Amanda S. Santos,5 Angeline M.S. Santos,23 María Sanz-Arnal,18 Giulia Melilli Serbin,27 Anádria Stéphanie da Silva,30 Rosemere dos Santos Silva,23 Andressa Dantas da Silveira,12 Graciele Lurdes Silveira,31 Gustavo
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