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BIBTEX RIS

Enterobacteria in anaerobic digestion of dairy cattle wastewater: Assessing virulence and resistance for one health security

2024; Elsevier BV; Volume: 252; Linguagem: Inglês

10.1016/j.watres.2024.121192

1879-2448

Kássia Vidal Menezes, Carlos Eduardo de Souza Duarte, Myleny Goularte Moreira, Talita de Jesus Cattem Moreno, Vitor José da Silva Pereira, João Gilberto Meza Ucella-Filho, Marcelo Henrique Otênio, Mariana Drummond Costa Ignacchiti, Juliana Alves Resende,

Gut microbiota and health

Samples from a dairy cattle waste-fed anaerobic digester were collected across seasons to assess sanitary safety for biofertilizer use. Isolated enterobacteria (suggestive of Escherichia coli) were tested for susceptibility to biocides, antimicrobials, and biofilm-forming capability. Results revealed a decrease in total bacteria, coliforms, and enterobacteria in biofertilizer compared to the effluent. Among 488 isolates, 98.12 % exhibited high biofilm formation. Biofertilizer isolates exhibited a similar biofilm formation capability as effluent isolates in summer, but greater propensity in winter. Resistance to biocides and antimicrobials varied, with tetracycline resistance reaching 19 %. Of the isolates, 25 were multidrug-resistant (MDR), with 64 % resistant to three drugs. Positive correlations were observed between MDR and increased biofilm formation capacity in both samples, while there was negative correlation between MDR and increased biocide resistance. A higher number of MDR bacteria were found in biofertilizer compared to the effluent, revealing the persistence of E. coli resistance, posing challenges to food safety and public health.