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PD53-07 INDUCED OBESITY CAUSES STRUCTURAL CHANGES IN THE TESTIS? AN EXPERIMENTAL MODEL

2024; Lippincott Williams & Wilkins; Volume: 211; Issue: 5S Linguagem: Inglês

10.1097/01.ju.0001009456.01770.91.07

1527-3792

Jorge Luiz Alves-Pereira, Luiz Carlos Schrotke Pires, Eliete Dalla Corte Frantz, D’Angelo Carlo Magliano, Luciano A. Favorito,

Hormonal and reproductive studies

You have accessJournal of UrologyInfertility: Basic Research & Pathophysiology (PD53)1 May 2024PD53-07 INDUCED OBESITY CAUSES STRUCTURAL CHANGES IN THE TESTIS? AN EXPERIMENTAL MODEL Jorge Luiz Alves Pereira, Luiz Carlos S. Pires, Eliete D. Frantz, Dangelo C. Magliano, and Luciano Alves Favorito Jorge Luiz Alves PereiraJorge Luiz Alves Pereira , Luiz Carlos S. PiresLuiz Carlos S. Pires , Eliete D. FrantzEliete D. Frantz , Dangelo C. MaglianoDangelo C. Magliano , and Luciano Alves FavoritoLuciano Alves Favorito View All Author Informationhttps://doi.org/10.1097/01.JU.0001009456.01770.91.07AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Obesity is a global health problem that has been linked to a variety of medical conditions, including hormonal and reproductive disorders, including changes in the testicles. Bisphenol S (BPS), which is widely used in the production of plastics and can be found in a variety of everyday products is associated with testis toxicity. The aim of this study is to evaluate the morphological and stereological parameters of the testicles in mice exposed to bisphenol S and/or high-fat diet-induced obesity. METHODS: Forty adult male C57BL/6 mice were fed a standard diet (standard show, SC) or high-fat diet (HF) for a total of 12 weeks. From the beginning of the experimental protocol, each group was randomly divided into 4 experimental groups with 10 mices as follows: a) SC - animals fed a standard diet; b) SC-B - animals fed a standard diet and administration of BPS (25 μg/kg of body mass/day) in drinking water; c) HF: animals fed a high-fat diet; d) HF-B - animals fed a high-fat diet and administration of BPS (25 μg/Kg of body mass/day) in drinking water. BPS administration lasted 12 weeks, following exposure to the SC and HF diets. BPS was diluted in absolute ethanol (0.1%) and added to drinking water at a concentration of 25 μg/kg body weight/day. At the end of the experiment, all animals were killed, and the testes were processed and stained with Masson's trichrome to for morphometric and stereological parameters, including density of seminiferous tubules per area, length density and total length of seminiferous tubules, height of the tunica albuginea and the diameter of the seminiferous tubules. The images were captured with an Olympus BX51 microscope and Olympus DP70 camera. The stereological analysis was done with the Image Pro and Image J programs. Means were statistically compared using ANOVA and the Holm-Sidak post-test (p<0.05). RESULTS: The seminiferous tubule density per area reduced in all groups when compared with SC samples (p<0.001): HF (40%), SC-B 3(2%), and HF-B (36%). Length density was reduced significantly (p<0.001) in all groups when compared with SC group: HF (40%), SC-B (32%), and HF-B (36%). The seminiferous tubule total length was reduced (p<0.001) when compared to f HF (28%) and SC-B (26%) groups. The tubule diameter increased significantly (p<0.001) only when we compare the SC group with SC (54%) an SC-B (25%) groups and the tunica thickness increased significantly only in HF group (117%) when compared with SC-B (20%) and HF-B 31%. CONCLUSIONS: Animals exposed to bisphenol S and/or high-fat diet-induced obesity presented important structural alterations in testicular morphology which suggest that this condition causes a deleterious effects in male genital system. Source of Funding: CNPQ and FAPERJ © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e1140 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Jorge Luiz Alves Pereira More articles by this author Luiz Carlos S. Pires More articles by this author Eliete D. Frantz More articles by this author Dangelo C. Magliano More articles by this author Luciano Alves Favorito More articles by this author Expand All Advertisement PDF downloadLoading ...