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MP65-07 DEVELOPMENT AND EVALUATION OF GEMCITABINE-LOADED LIPOSOMES IN AN ORTHOTOPIC MOUSE BLADDER CANCER MODEL

2024; Lippincott Williams & Wilkins; Volume: 211; Issue: 5S Linguagem: Inglês

10.1097/01.ju.0001008756.24343.22.07

1527-3792

ChungBeum Wee, Sejung Maeng, J.H. Tae, Se Young Choi, Mi‐Jeong Kim, Hak Jong Lee, Jong‐Hoon Kim, In Ho Chang,

Nanoplatforms for cancer theranostics

You have accessJournal of UrologyBladder Cancer: Basic Research & Pathophysiology III (MP65)1 May 2024MP65-07 DEVELOPMENT AND EVALUATION OF GEMCITABINE-LOADED LIPOSOMES IN AN ORTHOTOPIC MOUSE BLADDER CANCER MODEL ChungBeum Wee, Sejung Maeng, Jong Tae, Se Young Choi, Mi Jeong Kim, Hak Jong Lee, Jong Hoon Kim, and In Ho Chang ChungBeum WeeChungBeum Wee , Sejung MaengSejung Maeng , Jong TaeJong Tae , Se Young ChoiSe Young Choi , Mi Jeong KimMi Jeong Kim , Hak Jong LeeHak Jong Lee , Jong Hoon KimJong Hoon Kim , and In Ho ChangIn Ho Chang View All Author Informationhttps://doi.org/10.1097/01.JU.0001008756.24343.22.07AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: To overcome the limitation of conventional instillation, we developed gemcitabine-loaded liposomes (gem-liposomes) and investigated their antitumor effects in bladder cancer models. METHODS: Ultrasound-responsive liposomes were fabricated, and the conditions for loading gemcitabine into liposomes were established by adjusting the mass ratio of gemcitabine and liposomes, reaction temperature and time. We measured the size distribution and loading capacity (drug to lipid ratio) of gem-liposomes and evaluated in vitro release of gemcitabine from gem-liposomes under ultrasound exposure. We decided proper dose of gem-liposomes according to the effects of at bladder cancer cell line (T24, 253J and 5637) spheroid models. And then we evaluated the antitumor effects of gem-liposomes intravesical instillation at orthotopic mouse bladder cancer model using MBT2 cell lines. RESULTS: The gem-liposomes showed a size distribution of 104.9±31.7 nm, and the loading capacity of gemcitabine was 15.17% (w/w%). The release of gemcitabine under the ultrasound irradiation was 62.5±6.38%. In 2D and 3D spheroid model of bladder cancer, gem-liposomes treatment showed antitumor effects in T24, 253J and 5637 cell lines compared to conventional gemcitabine without any difference. (However, cell viability after ultrasound exposure show the anti-tumor effects dose-dependently by gem-liposomes.) In orthotopic mouse bladder cancer, intravesical instillation of gem-liposome showed more antitumor effects than conventional intravesical instillation of gemcitabine, but there was no difference in body weights and survival rates between gem-liposome and conventional gemcitabine groups. CONCLUSIONS: We developed a gem-liposome formulation to overcome the rapid washout phenomenon, as a disadvantage of conventional gemcitabine, and to maximize therapeutic efficacy. Gem-liposomes improved therapeutic efficacy in an in vivo bladder cancer model by increasing the drug delivery efficacy due to the long-lasting effect near the tumor and the slow release of the drug from the gem-liposome, which is characteristic of ultrasound-responsive liposomes. The new concept of ultrasound-responsive gemcitaibine formulation described in this study represents a new treatment paradigm in NMIBC treatment. Download PPT Source of Funding: None © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e1079 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information ChungBeum Wee More articles by this author Sejung Maeng More articles by this author Jong Tae More articles by this author Se Young Choi More articles by this author Mi Jeong Kim More articles by this author Hak Jong Lee More articles by this author Jong Hoon Kim More articles by this author In Ho Chang More articles by this author Expand All Advertisement PDF downloadLoading ...