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Chemical Control of Fluorescence Lifetime towards Multiplexing Imaging

2024; Wiley; Volume: 63; Issue: 25 Linguagem: Inglês

10.1002/anie.202403029

1521-3773

Junbao Ma, Feng Luo, Chia‐Heng Hsiung, Jianan Dai, Zizhu Tan, Songtao Ye, Lina Ding, Baoxing Shen, Xin Zhang,

Photoreceptor and optogenetics research

Abstract Fluorescence lifetime imaging has been a powerful tool for biomedical research. Recently, fluorescence lifetime‐based multiplexing imaging has expanded imaging channels by using probes that harbor the same spectral channels and distinct excited state lifetime. While it is desirable to control the excited state lifetime of any given fluorescent probes, the rational control of fluorescence lifetimes remains a challenge. Herein, we chose boron dipyrromethene (BODIPY) as a model system and provided chemical strategies to regulate the fluorescence lifetime of its derivatives with varying spectral features. We find electronegativity of structural substituents at the 8′ and 5′ positions is important to control the lifetime for the green‐emitting and red‐emitting BODIPY scaffolds. Mechanistically, such influences are exerted via the photo‐induced electron transfer and the intramolecular charge transfer processes for the 8′ and 5′ positions of BODIPY, respectively. Based on these principles, we have generated a group of BODIPY probes that enable imaging experiments to separate multiple targets using fluorescence lifetime as a signal. In addition to BODIPY, we envision modulation of electronegativity of chemical substituents could serve as a feasible strategy to achieve rational control of fluorescence lifetime for a variety of small molecule fluorophores.