Portal de Periódicos da CAPES

Co-occurrence of triple carbapenemase genes, bla VIM-2 , bla NDM-1 , and bla OXA-48 in Enterobacter hormaechei clinical isolates –first report from Croatia

2024; Taylor & Francis; Linguagem: Inglês

10.1080/1120009x.2024.2354107

1973-9478

Zrinka Bošnjak, Henrik Hasman, Frank Hansen, Anette M. Hammerum, Louise Roer, Ivana Jurić, Ana Budimir,

Genomics and Phylogenetic Studies

Two Enterobacter hormaechei isolates harbouring three carbapenemase genes each, were isolated from two patients from different ICUs at University Hospital Centre Zagreb, Croatia, which is to our knowledge, the first report of triple carbapenemase (blaVIM-2, blaNDM-1, and blaOXA-48) co-existence in E. hormachei strains and also among Enterobacterales members in Croatia. Antimicrobial susceptibility testing showed susceptibility only to colistin and amikacin. The production of carbapenemases was phenotypically tested by immunochromatographic assay and confirmed by PCR. Detailed analysis by Whole Genome Sequencing (WGS) of short reads by Illumina and long reads by Oxford Nanopore Technologies (ONT) was additionally performed and showed that both isolates belonged to ST200. They were separated by 98 Single Nucleotide Polymorphisms (SNPs) having variations in the number of blaVIM-2 genes on the chromosome, the number of blaNDM-1 genes on the plasmid, non-identical blaNDM-1 plasmids, different plasmid content in general, and only one isolate carried a 94 kb prophage.