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Comparison of P-glycoprotein gene expression of two Haemonchus contortus isolates from Yucatan, Mexico with resistance and susceptible phenotype to ivermectin in relation a susceptible reference strain

2024; Elsevier BV; Volume: 52; Linguagem: Inglês

10.1016/j.vprsr.2024.101047

2405-9390

Lisandro Alberto Encalada-Mena, J.F.J. Torres-Acosta, C.A. Sandoval-Castro, David Emanuel Reyes-Guerrero, Gabriela Mancilla-Montelongo, Raquel López‐Arellano, Agustín Olmedo-Juárez, M.E. López-Arellano,

Parasite Biology and Host Interactions

The variability in the expression of different P-glycoprotein (P-gp) genes in parasitic nematodes of ruminants such as Haemonchus contortus (Hco-pgp) may be caused by different factors including nematode biology, geographical region and anthelmintic pressure. This study analysed the relative expression level of 10 P-gp genes in two H. contortus (Hco-pgp) field isolates from Yucatan, Mexico: 1) PARAISO (IVM-resistant) and 2) FMVZ-UADY (IVM-susceptible). These isolates were compared with a susceptible reference isolate from Puebla, Mexico, namely "CENID-SAI". In all cases H. contortus adult males were used. The Hco-pgp genes (1, 2, 3, 4, 9, 10, 11, 12, 14 and 16) were analysed for each isolate using the RT-qPCR technique. The Hco-pgp expressions were pairwise compared using the 2