E‐Posters
2024; Wiley; Volume: 36; Issue: S1 Linguagem: Português
10.1111/den.14865
ISSN1443-1661
Autores ResumoDigestive EndoscopyVolume 36, Issue S1 p. 102-376 AbstractFree Access E-Posters First published: 02 July 2024 https://doi.org/10.1111/den.14865AboutSectionsPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat EPOSTER AREA: 1. ADVANCED/EXPERIMENTAL MUCOSAL IMAGING P001 DIAGNOSTIC PERFORMANCE OF MAGNIFYING LINKED COLOR IMAGING VERSUS MAGNIFYING BLUE LASER IMAGING FOR IDENTIFYING THE INVASION DEPTH OF SUPERFICIAL ESOPHAGEAL SQUAMOUS CELL CARCINOMA Z. Wang, L. Li, E. Linghu, N. Chai Department of Gastroenterology, First Medical Center of Chinese PLA General Hospital, Beijing, China AIMS: We aim to evaluate the diagnostic accuracy of invasion depth in magnifying linked color imaging (M-LCI) compared with magnifying blue light imaging (M-BLI). METHODS: In this prospective study, we enrolled 58 patients who underwent magnifying endoscopy in the First Medical Center of Chinese PLA General Hospital from February 2022 to September 2023. M-LCI and M-BLI observed the suspicious esophageal lesions, respectively. The neoplastic lesions and their invasion depth were evaluated according to the microvascular morphological classification proposed by the Japan Esophageal Society. The histopathological results were used as the gold standard, and we compared the diagnostic accuracy of the two methods. RESULTS: A total of 58 patients (77 lesions) were biopsied under the magnification endoscopy, among which 49 lesions were pathologically confirmed as inflammation and LGIN, 9 lesions as HGIN, 8 lesions as invasion to the epithelium or lamina propria mucosa (EP/LPM), 10 lesions as invasion to the lamina muscularis mucosa or SM1, and 1 lesion as invasion ≥SM2. There were no statistical differences between M-LCI and M-BLI in diagnosing ESCC and HGIN. The overall accuracy of M-LCI and M-BLI in identifying the invasion depth was 64.3% and 75.0%, respectively (P = 0.562). The diagnostic accuracy of M-LCI and M-BLI was comparable according to the subgroup analysis of different invasion depths. CONCLUSIONS: M-LCI has a certain value in identifying the tumor invasion depth through microvascular classification, and the diagnostic accuracy is comparable to M-BLI, both of which can be used in the diagnosis and prediction of early esophageal squamous cell carcinoma. P002 OBJECTIVE ANALYSIS OF LINKED COLOR IMAGING AND TEXTURE COLOR ENHANCEMENT IMAGING IN THE EVALUATION OF ENDOSCOPIC FINDINGS OF MAP-LIKE REDNESS T. Takeda, T. Iwano, M. Yamamoto, Y. Uemura, R. Uchida, H. Utsunomiya, D. Abe, N. Suzuki, S. Oki, A. Ikeda, Y. Akazawa, K. Ueda, H. Ueyama, D. Asaoka, M. Hojo, A. Nagahara Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan AIMS: Linked Color Imaging (LCI) and Texture and color enhancement imaging (TXI), newly developed for image-enhanced endoscopy, allows the easy recognition of differences in mucosal color and structure. The purpose of this study was to evaluate the color differences of endoscopic findings of map-like redness using LCI or TXI. METHODS: A single-center retrospective clinical study was performed to investigate whether the color differences of the endoscopic findings of map-like redness according to the Kyoto Classification of Gastritis improved using LCI or TXI compared with white light imaging (WLI). Patients who underwent esophagogastroduodenoscopy with WLI and LCI or TXI (mode1: TXI-1, mode2: TXI-2) from March 2016 to March 2022 at our hospital were enrolled. Images were objectively evaluated based on L* a* b* color values and color differences (ΔE*) between surrounding gastric mucosa and map-like redness in a CIELAB color space system. RESULTS: Forty-two cases of map-like redness with LCI and 40 cases with TXI were included in this study. The a* values of map-like redness showed WLI: 46.2, LCI: 42.4 (WLI vs LCI: P = 0.01), WLI: 47.5, TXI-1: 50.3, TXI-2: 52.5 (WLI vs TXI-1: P = 0.04, WLI vs TXI-2: P = 0.03). The b* values of map-like redness showed WLI: 45.5, LCI: 23.4 (WLI vs LCI: P < 0.01), WLI: 41.9, TXI-1: 35.6, TXI-2: 42.0 (WLI vs TXI-1: P < 0.01, WLI vs TXI-2: P = 0.87). The color difference (ΔE*) revealed WLI: 13.8, LCI: 30.7 ± 6.7 and WLI: 13.6 ± 5.4, TXI-1: 26.6 ± 7.5, TXI-2: 20.0 ± 6.4. Statistically significant difference between WLI and LCI, WLI and TXI-1, WLI and TXI-2, and TXI-1 and TXI-2 was observed (P < 0.01). CONCLUSIONS: Both LCI and TXI provide better contrasting images between surrounding gastric mucosa and map-like redness. DISCLOSURE: A clinically commissioned/joint research grant (Olympus Co., Ltd). A joint clinical research grant from Fujifilm Co., Ltd. P003 CONCORDANCE BETWEEN NICE CLASSIFICATION AND HISTOPATHOLOGY IN COLONIC POLYPS: A TERCIARY CENTER EXPERIENCE C.E. Lombo-Moreno1, A.C. Buitrago-Tamayo1, A.M. Leguizamo-Naranjo1, O.M. Muñoz-Velandia2, R.D. Vargas-Rubio1 1Gastroenterology, Pontificia Universidad Javeriana, Bogota, Colombia, 2Internal Medicine, Pontificia Universidad Javeriana, Bogota, Colombia AIMS: To evaluate concordance between NICE classification and histopathology in a reference hospital in Colombia. METHODS: Prospective analytic study performed in Hospital Universitario San Ignacio (Colombia) between 2021 and 2022. Concordance between NICE I, II and III classification and histopathology (a. Hyperplastic Polyp (HP)/Sessile Serrated Polyp (SSP) b. Adenoma; and c. Deep Submucosal Invasive Cancer (DSIC), respectively) was evaluated using weighted kappa. Diagnostic performance was evaluated between NICE I-II vs NICE III against (adenoma/HP/SSP) vs DSIC. Subgroup analysis was performed for polyps ≥10 mm and those located in rectum, sigmoid and left colon (categorized as left sided polyps). Institutional research committee approved this study (FM-CIE-0007-22). RESULTS: 238 polyps from 135 patients were evaluated. Median age 67 years (IQR 58.5–74.5), 54.4% males. 23 (17.1%) had ≥3 polyps. 124 (52.1%) polyps were located on rectum, sigmoid and left colon. 182 (76.5%) were <10 mm. NICE and histopathology evidenced a fair-moderate concordance (Quadratic weighted kappa 0.36, linear weighted kappa 0.43). NICE classification (NICE I-II vs III) compared to histopathology (DSIC vs adenoma/HP or SPP) evidenced a sensitivity of 90.9% and specificity of 99.1%. NICE III specificity for DSIC diagnosis was ≥95% on polyps ≥10 mm and for left sided polyps. CONCLUSIONS: NICE and histopathology concordance is suboptimal. However, NICE III concordance for DSIC diagnosis evidenced good specificity. Therefore, NICE III polyps require a prompt histopathological evaluation and follow-up. Good operative characteristics stand in polyps ≥10 mm and left sided polyps. NBI formal training is recommended in gastroenterology units in South America. P004 CLINICAL IMPACTS OF THE JES-BE MAGNIFYING ENDOSCOPIC CLASSIFICATION ON ENDOSCOPICALLY RESECTED SUPERFICIAL BARRETT'S ADENOCARCINOMAS Y. Kawata, K. Goda, M. Ishikawa, S. Furui, K. Oike, S. Yamaguchi, T. Yoshinaga, M. Kondo, A. Kanamori, K. Abe, T. Sugaya, K. Tominaga, A. Irisawa Department of Gastroenterology, Dokkyo Medical University, Tochigi, Japan AIMS: The objective of the present study was to identify the impacts of narrow-band imaging magnifying endoscopy (NBI-ME) based on JES-BE classification on the diagnostic accuracy of preoperative inspection for lateral tumor extent and treatment outcomes of superficial Barrett's adenocarcinomas (SBA). METHODS: The subjects were 15 patients (16 lesions) diagnosed with SBA that occurred in the short-segment Barrett's esophagus (SSBE) or long-segment Barrett's esophagus (LSBE) and on which endoscopic submucosal dissection (ESD) was performed at Dokkyo Medical University Hospital, between September 2018 and August 2023. All cases underwent NBI-ME using JES-BE classification before ESD. Diagnostic accuracy of the NBI-ME for lateral tumor extent (LTE) and outcomes of ESD were evaluated and compared between the SSBE group (SSBE-G, n = 10) and the LSBE group (LSBE-G, n = 6). RESULTS: Majority of patients was men and mean age was over 65 years in both SSBE-G and LSBE-G. Main macroscopic type of 0-IIb (completely flat type) were more frequently observed in LSBE-G than in SSBE-G. 0-IIb comportments were found in 100% of LSBE-G, which was significantly higher than SSBE-G (P = 0.008). The mean tumor diameters were significantly larger in LSBE-G than SSBE-G (45 mm vs. 15 mm: P < 0.001). Diagnostic accuracy of LTE using white light imaging (WLI) alone was 70% in SSBE-G, but improved it 100% by NBI-ME using the JES-BE classification. In LSBE-G, the diagnostic accuracy of LTE was 0% when using WLI alone, but this improved to 100% by NBI-ME. The recurrence rate in the remnant mucosa of Barrett's esophagus was 0% in both groups in the mean follow-up period of 18 (13–27) months after ESD. CONCLUSIONS: NBI-ME using the JES-BE classification could improve diagnostic accuracy of the lateral tumor extent of SBAs especially in the LSBE, and may provide favorable outcomes of ESD therapy. P005 A COMPARATIVE STUDY ON IMAGE ENHANCED ENDOSCOPY FOR THE DETECTION OF GASTRIC SUPERFICIAL NEOPLASIA: LINKED COLOR IMAGING VERSUS WHITE LIGHT IMAGING S.P. Lee1, J.G. Lee2, I.K. Yoo3 1Hanyang University College of Medicine, Internal Medicine, Seoul, Republic of Korea, 2Hallym University Dongtan Sacred Heart Hospital, Internal Medicine, Hwaseong, Republic of Korea, 3CHA Bundang Medical Center, Internal Medicine, Seongnam, Republic of Korea AIMS: Early detection of gastric superficial neoplasia (GSN) through screening endoscopy is very important, but endoscopic detection and diagnosis are sometimes difficult. Linked color imaging (LCI) can help reduce the miss rate of GSN and increase tumor detection rate compared with white light imaging (WLI). We aimed to investigate whether LCI is superior to WLI in detecting and diagnosing new GSNs in screening endoscopy. METHODS: This randomized multi-center prospective study was conducted on patients with suspected or diagnosed gastric adenoma or early gastric cancer. Endoscopic evaluations in the LCI group were performed under LCI mode, and those in the WLI group were done by WLI mode. After the first observation was completed in each group, additional observation was performed in a different mode. The miss rate of GSN, tumor detection time, and tumor characteristics were evaluated. RESULTS: 48 patients (52 tumors) and 47 (52 tumors) belonged to the LCI and WLI groups. The miss rates of GSN were 5.77% and 13.46% for the LCI and WLI groups, respectively. The tumor detection time in the LCI group was significantly shorter than that in the WLI group (P = 0.049). Tumors not detected in the first observation were significantly more likely to be IIb morphology than those that were (P = 0.014). CONCLUSIONS: LCI may help reduce the miss rate of GSN and enable faster detection. IIb tumors may be more difficult to detect endoscopically than other tumors. P006 USEFULNESS OF MAGNIFYING ENDOSCOPY WITH NARROW-BAND IMAGE (MENBI) FOR PREDICTING THE PRESENCE OF UNDIFFERENTIATED COMPONENT IN EARLY GASTRIC CANCER J.H. Kim, J. Seo Dr. Seo's Medical Clinic, Departments of Internal Medicine, Changwon, Republic of Korea AIMS: As undifferentiated (UD) early gastric cancer (EGC) carries a high risk of micro-invasion, lymph node metastasis and non-curative resection, identifying the predictive factors for presence of UD component is of clinical important for decision of treatment strategy for EGC. The aim of this study was to investigate the demographic, endoscopic and magnifying endoscopy with narrow band image (MENBI) features for predicting the presence of UD component in EGC. METHODS: Demographic, endoscopic features of 184 consecutive EGC patients who underwent MENBI examination and endoscopic submucosal dissection (ESD) were retrospectively analyzed. Cases with of histologic component of signet-ring cell carcinoma, poorly differentiated adenocarcinoma and mucinous adenocarcinoma on post-ESD histology were classified as undifferentiated EGC (UD-EGC) group. RESULTS: 56 among total 184 cases (30.4%) discovered as UD-EGC group. UD-EGC showed significantly higher rate of non-curative resection compared to differentiated EGC (D-EGC) group. Age (≤60) (P = 0.028), Proximal location (P = 0.005), larger size (≥3 cm) (P = 0.027), presence of endoscopic ulcer or ulcer scar (P = 0.001), ill-defined endoscopic margin (P < 0.001) and absent microsurface pattern (MSP) on MENBI examination (P < 0.001) were significantly related to presence of UD component in the univariate analysis. Among those, ill-defined endoscopic margin (P = 0.001, OR = 6.59, 95% CI: 2.186–19.836) and presence of absent MSP pattern on MENBI examination (P < 0.001, OR = 17.164, 95% CI: 6.088–48.392) were determined to be significant according to a multivariate analysis. CONCLUSIONS: EGC with ill-defined endoscopic margin combined with absent MSP on MENBI finding carry a high risk of presence of undifferentiated component. P007 NARROW BAND-IMAGING VERSUS HISTOPATHOLOGY IN DIFFERENTIATION BETWEEN ADENOMATOUS AND NON-ADENOMATOUS COLORECTAL POLYPS M.L. Asser1, S.A. Ooda1, S.A. Lashen2, M.Y. Alhassafi2, A.S. Alsedefy3 1Department of Clinical and Experimental Internal Medicine, Medical Research Institute, Alexandria University, Alexandria, Egypt, 2Faculty of Medicine, Department of Internal Medicine, Alexandria University, Alexandria, Egypt, 3Department of Pathology, Medical Research Institute, Alexandria University, Alexandria, Egypt AIMS: As colorectal polyps represent the precursors for colorectal cancer, early detection and removal of polyps during screening colonoscopy reduces the incidence of colorectal cancer and its related mortality. As the polyp histology cannot be differentiated reliably using the standard white-light colonoscopy, all polyps detected during colonoscopy should be removed and examined histologically. This has many drawbacks including risk of bleeding, perforation and the cost burden of the unnecessary removal of non-neoplastic polyps. Digital chromoendoscopy is a novel technology that have emerged and enabled detailed visualization of the colonic mucosa and in-vivo characterization of morphology and types of colorectal polyps, and thus the need of their removal. Our study aimed at the use of Narrow-Band Imaging assisted colonoscopy in differentiation between adenomatous and non-adenomatous colorectal polyps and comparing its accuracy to histopathology. METHODS: Prospectively, we included 28 patients at Faculty of Medicine, Alexandria University, Egypt in the period between November 2021 and August 2022 who underwent colonoscopy having 38 colorectal polyps which were characterized by Narrow-Band Imaging International Colorectal Endoscopic classification, removed endoscopically and histologically evaluated. RESULTS: The study proved a good correlation and a high degree of agreement between endoscopic Narrow-Band Imaging and histological diagnosis of polyps [P < 0.001, Kappa κ = 0.803]. CONCLUSIONS: Narrow-Band Imaging assisted colonoscopy could be used as a reliable method for in-vivo detection of colorectal adenomas and their removal. Also, it proved the accuracy and validity of Narrow-Band Imaging International Colorectal Endoscopic classification in differentiation between adenomatous and non-adenomatous polyps. P008 EFFICACY OF EXTENDED DEPTH OF FIELD (EDOF) ENDOSCOPE FOR DIFFERENTIATION OF COLORECTAL DIMINUTIVE POLYPS M. Fukuda, H. Horiuchi, N. Tada, M. Ito, Y. Hasegawa, T. Futakuchi, N. Tamai, K. Sumiyama The Jikei University School of Medicine, Tokyo, Japan AIMS: Developing a modality with high diagnostic ability for small colonic lesions (less than 5 mm) is an important challenge. The purpose of this study is to clarify the usefulness of qualitative diagnosis using an endoscope equipped with the Extended Depth of Field (EDOF) function. METHODS: Patients scheduled for endoscopic treatment of known colonic lesions at our hospital from June to September 2023 were enrolled. Qualitative diagnosis of colonic lesions less than 5 mm detected by white light observation was performed by EDOF-NBI observation, followed by qualitative diagnosis by white light observation, and the lesions were removed endoscopically. The diagnostic ability of EDOF-NBI observation and white light observation for qualitative diagnosis was prospectively evaluated. RESULTS: A total of 100 lesions in 29 patients were evaluated during the study period. The age of the patients was 62.3 ± 12.1 years, and the male-to-female ratio was 73:27. The localization of the target lesions was as follows: 1 lesion (1%) in the cecum, 28 lesions (28%) in the ascending colon, 29 lesions (29%) in the transverse colon, 5 lesions (5%) in the descending colon, and 27 lesions (27%) in the rectum and sigmoid colon. The macroscopic type was 0-Is in 59 lesions (59%) and 0-IIa in 41 lesions (41%). The pathological diagnosis revealed that 70 lesions (70%) were tumors and 30 lesions (30%) were non-tumors. The diagnostic performance of EDOF-NBI observation for neoplastic lesions was 87.5% sensitivity, 75.0% specificity, 72.7% negative predictive value, and 90.0% positive predictive value for all colorectal lesions, and that of white light observation was 88.0% sensitivity, 71.0% specificity, 72.7% negative predictive value, and 87.1% positive predictive value. The area under the receiver operating characteristic curve was 0.800 for EDOF-NBI observation and 0.8024 for white light observation. CONCLUSIONS: The usefulness of qualitative diagnosis using EDOF-NBI observation for small colonic lesions was demonstrated. P009 A NOVEL MAGNIFYING ENDOSCOPIC FINDING OF ANNULARLY ARRANGED TYPE A VESSELS IN SUPERFICIAL ESOPHAGEAL SQUAMOUS CELL CARCINOMAS: A CASE SERIES STUDY FOR THE HISTOPATHOLOGICAL FEATURES AND UTILITY OF ENDOCYTOSCOPY K. Goda1, K. Ishida2, M. Kondo1, K. Abe1, M. Ishikawa1, S. Yamaguchi1, K. Yosuke1, T. Yoshinaga1, T. Sugaya1, K. Tominaga1, H. Masuyama3, A. Irisawa1 1Department of Gastroenterology, Dokkyo Medical University School of Medicine, Tochigi, Japan, 2Diagnostic Pathology, Dokkyo Medical University School of Medicine, Tochigi, Japan, 3Masuyama Gastrointestinal Clinic, Tochigi, Japan AIMS: The Japan Esophageal Society (JES) classification, which systematizes morphological changes in the surface microvasculature as observed by magnifying endoscopy in regional esophageal lesions, has come into widespread use for its utility in the diagnosis of superficial esophageal squamous cell carcinoma (SCC). In the JES classification, the microvessel morphology is classified as either Type A (non-cancerous) or Type B (cancerous). However, we experienced five SCCs invading to the lamina propria mucosae (LPM) which had only Type A vessels and partially showing annularly arranged (i.e. annularly arranged Type A (AAA) vessels). This study aimed to investigate the clinicopathological characteristics including endocytoscopy (EC) in the five invasive SCCs. METHODS: Between December 2018 and November 2023, endocytoscopy (EC) was performed at Dokkyo Medical University Hospital for three cases (five lesions) found to have AAA vessels using narrow-band imaging magnifying endoscopy (NBI-ME). EC findings were determined using the Modified EC classification, and all of the lesions were resected by endoscopic submucosal dissection (ESD). The ESD specimens were prepared with serial sections and inspected using inmmunohistochemistory (p53, Ki-67, CD34, and D2-40). RESULTS: All three cases comprised male patients with a mean age of 64 years. All of the lesions were flat (0–IIb) and measured ≤6 mm in diameter and were detected as brownish areas by NBI. EC showed findings corresponded to EC2 (borderline lesions)/3 (SCC) or EC3 in all lesions. Histologically, all lesions showed SCCs invading to the LPM, and four (80%) lesions were basal-layer-type SCCs. In the three (60%) lesions, AAA vessels were located in in the invading cancer nests. CONCLUSIONS: AAA vessels by NBI-ME could be suggestive of not non-cancerous lesions but T1a-LPM SCCs even if the lesions did not have any Type B vessels. EC may be useful in the histologic prediction of the small lesions with the unique findings. P010 ENDOSCOPIC PREDICATION OF INVASIVE DEPTH IN EARLY GASTRIC CANCER BY MAGNIFYING ENDOSCOPY WITH NARROW-BAND IMAGING: A PILOT FEASIBILITY TRIAL C.H. Oh1, J.-Y. Jang2, J.-W. Kim3 1Kyung Hee University College of Medicine, Department of Gastroenterology and Hepatology, Seoul, Republic of Korea, 2Kyung Hee University College of Medicine, Department of Internal Medicine and Hepatolgy, Seoul, Republic of Korea, 3Kyung Hee University School of Medicine, Department of Gastroenterology and Hepatology, Seoul, Republic of Korea AIMS: Conventional white light endoscopy is suboptimal for the evaluation of depth of invasion in early gastric cancer (EGC). The aim of this study was to estimate the accuracy and feasibility of a simplified magnifying NBI (M-NBI) classification for predicting invasion depth of early gastric cancer. METHODS: A total of 42 consecutive patients undergoing M-NBI before endoscopic or surgical resection for EGC were included. Previously proposed M-NBI features were analyzed in order to assess feasibility and accuracy in real practice. We classified NBI findings into three categories concisely according to surface and vascular patterns as follows: abnormally dilated and tortuous blood vessels (Type 1), sparse and irregular microvessels (Type 2), and avascular areas (Type 3) (Fig. 1). RESULTS: Of 42 patients, 14 (33.3%) had submucosal cancer based on final pathologic results. Although the sensitivity of each category (type 1–3) was low (22.2%, 44.4%, and 66.7%), specificity was relatively high (65.5%, 82.9%, and 93.1%). Likewise, negative predictive values of each category were high (93.4%, 94.6%, and 95.9%). CONCLUSIONS: Although the accuracy of M-NBI for the prediction of depth of invasion showed disappointing results, it is likely to have the potential to discriminate mucosal cancer before endoscopic resection of EGC. P011 HYPERPLASIA OR SSL POLYP UPON DECISION IN REAL TIME:AN ENDOCYTOSCOPIC EXAMINATION CASE F. Zhang, B. Shi, H. Xu Endoscopy Centre, First Hospital of Jilin University, Changchun, China AIMS: A screening case justified that Endocytoscopy is more detail-providing to endoscopists in clinic decision in real time. METHODS: Patient Ms Cao, Female, 38 yo. Intermittent pasty stool for half a year, without fresh blood stool. No significant weight loss, no significant past history and no Family history. A screening colonoscope with GIF-H290EC (Olympus) was set for her examination. RESULTS: Two sessile polyps were found by colonoscope, one is mild elevated lesion in cecum. It was observed undre NBI mode with magnified function diagnosed as NICE1, JNET1, and PitPattern II-O, BUT after methlyn blue dye, it showed EC type was EC1b. the other was a mild elevated lesion in transverse colon with gray color (0-IIa, 1.0CMx1.2CM), under NBI with magnified observing concluded that it was NICE-1 JNET-1, EC-V1 with methlyn blue dye. The small lesion with more aggressive morphologic changes was diagnosed by EC endoscopy. After EMR en bloc rescetion, the pathology confirmed EC results that the cecum one is SSL is low grade intraepithelial neoplasia, but hte larger one in transverse colon is just hyperplasia polyp. CONCLUSIONS: Endocytoscopy is more powerful than chromoscopy and magnified endoscopy to providing more detail and accurate diagnosis in this case. P012 MULTIMODAL MULTIPHOTON MICROIMAGING IN THE DETECTION OF GASTRIC NEOPLASTIC LESION X. Wang1, J. Wu2, Y. Xing3, X. Sui1, M. Wang1, L. Li1 1Department of Gastroenterology, Strategic Support Force Medical Center, Beijing, China, 2Department of Pathology, Strategic Support Force Medical Center, Beijing, China, 3Key Laboratory of Optical Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, China AIMS: We attempted to explore the role of two-photon excitation fluorescence (TPEF), three-photon excitation fluorescence (TrPEF), second harmonic generation (SHG), and third harmonic generation (THG) imaging in gastric cancer research. METHODS: A total of 7 gastric cancer, 7 normal stomach, and 5 chronic gastritis with atrophy/intestinal metaplasia tissue samples were included. Two consecutive slices were selected, one for MPM imaging and the other stained with Hematoxylin–Eosin (H&E) and analyzed by experienced pathologists. Based on a Yb-fiber laser that can produce 990-nm and 1110-nm pulse. The two wavelength pulses were utilized to drive TPEF/SHG and TPEF/TrPEF/SHG/THG for gastric tissue imaging, respectively. The MPM images were compared with corresponding H&E images. The nuclear areas of 50 randomly selected epithelial cells from each region were measured by ImageJ. The results were expressed as mean ± SD and statistical analysis was performed using SPSS 22.0. P < 0.05 was considered statistically significant. RESULTS: The MPM images were highly consistent with the images of corresponding locations of H&E stained slices. Four-layer structures of gastric wall were clearly shown in normal and atrophy/intestinal metaplasia stomach. Mucosal epithelial cells are uniform in size and well-arranged, with consistently sized nuclei. The gastric glands were arranged regularly. In gastric cancer tissue glands were disorganized. The size of epithelial cells varied, with nuclei of different sizes and diverse morphologies. The extracellular matrix was chaotically arranged, collagen fibers were sparse and fractured, and the tumor invaded into muscle layer. The average nuclear area of mucosal epithelial cells in normal mucosa, mucosa of atrophy/intestinal metaplasia, and gastric cancer tissue was 43.84 ± 8.30 μm2, 42.93 ± 9.17 μm2, and 76.90 ± 18.36 μm2, respectively, with P < 0.001, indicating statistically significant differences. CONCLUSIONS: Based on this novel wavelength conversion technology, TPEF/TrPEF/SHG/THG imaging of gastric tissue can be performed, and gastric cancer tissue and non-cancerous tissue can be distinguished by qualitative and quantitative analysis. P013 BIOLOGICAL POTENTIAL OF CURCUMIN AS A PROMISING ANTICANCER AGENT FOR THE TREATMENT OF ACUTE MYELOID LEUKEMIA (AML) THROUGH ITS CHEMOPREVENTION AND OTHER MOLECULAR MECHANISM D.K. Patel Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, India AIMS: Leukemia is malignant proliferations of leukocytes. Acute myeloid leukemia (AML) is characterized as the most common form of leukemia in the adult population, which affects a million individuals and leads many deaths worldwide. Medicinal plants contain numerous active phytochemical called secondary metabolites that have therapeutic value in medicine for the treatment of human diseases. Curcumin is an important class of phytochemical of Curcuma longa and principal curcuminoid of Curcuma longa, having numerous applications in herbal, cosmetics, and food industries. METHODS: The present study identified the biological potential and therapeutic effectiveness of curcumin against AML. Biological potential of curcumin have been investigated in the present work through its cytotoxic effects on leukemia cells. Further, biological potential of curcumin on ALDH+ cells in the THP-1 cell line has also been investigated in the scientific field. Further, its effectiveness on mRNA levels of the signal transducer and activator of transcription 3 (STAT3) has also been investigated in some other scientific work. Other pharmacological activities of curcumin have also been investigated in the present work in order to know its effectiveness on AML. RESULTS: Biological potential of curcumin on ALDH+ cells in the THP-1 cell line has been investigated in the scientific field and signified their effectiveness on ALDH+ cells in the THP-1 cell line, as curcumin significantly decreased the population. Curcumin have been investigated for its cytotoxic effects on leukemia cells and found to have significant therapeutic potential. Curcumin was found to decrease the mRNA levels of the signal transducer and activator of transcription 3 (STAT3). However, in another scientific study, combination of curcumin with some carrier's molecules also improved the survival rate of mice with AML. Other scientific data also signified the therapeutic effectiveness of curcumin on AML. CONCLUSIONS: Present study signified the biological importance of curcumin in medicine for their effectiveness on AML. P014 AUTOFLUORESCENT DIAGNOSTICS OF EPITHELIAL FORMATIONS DURING ENDOSCOPIC EXAMINATIONS OF THE STOMACH V. Duvanskiy, M. Knyazev, A. Belkov Endoscopy, Endoscopic and Laser Surgery, RUDN University, Moscow, Russian Federation AIMS: To find out the correlation between autofluorescence (AF) staining of gastric neoplasms and their histomorphological structure. METHODS: Esophagogastroduodenoscopy revealed gastric epithelial formations in 123 patients, aged 22–83 (52 ± 5.9); males – 46, females – 77. Olympus Lucera CV-260 endoscopic video system with AFI autofluorescence mode was used. AF staining of formations in purple or green was recorded. So as to clarify the correlation between the type of AF staining of gastric epithelial formations and their histological structure, all formations were divided into four groups according to the Vienna Classifica
Referência(s)