Produção Nacional
Synthetic Tetraploid of Oncidium crispum Lodd. (Orchidaceae)
2024; Springer Science+Business Media; Linguagem: Inglês
10.1007/978-1-0716-3954-2_18
ISSN1940-6029
AutoresThalita Bordignon da Cunha, Saulo Marçal de Sousa, Aryane Campos Reis, Elyabe Monteiro de Matos, Lyderson Fácio Viccini,
Tópico(s)Plant tissue culture and regeneration
ResumoIn ornamental plants, artificial polyploidization has enabled the creation of new cultivars. Due to their high commercial value in the international flower market and their ornamental characteristics, such as the shape, size, color, and durability of their flower, orchids have received great attention in studies of artificial polyploidization. Here we described the protocol used for polyploid induction in Oncidium crispum, an epiphyte species native of southeastern Brazil, of great ornamental interest and widely sold in flower shops. The species stands out for having inflorescence with large flowers, brown in color with yellow spots. In addition, O. crispum has great potential for use in genetic improvement programs since the species is widely used in interspecific crosses. Closed capsules containing mature O. crispum seeds were subjected to running sterilized water for 10 min and then to a 1.5% sodium hypochlorite solution for 10 min. Small portions of seeds were introduced into 50 mL of water-soluble fertilizer with macro- and micronutrients (B&G
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