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Treatment of cryopreserved bovine sperm with calcium ionophore A23187 increases in vitro embryo production

2024; Elsevier BV; Volume: 229; Linguagem: Inglês

10.1016/j.theriogenology.2024.08.009

1879-3231

Claudia Osycka‐Salut, Magalí Waremkraut, R. Garaguso, E. Piga, E. Martínez-León, Clara I. Marín‐Briggiler, Marıá Gracia Gervasi, Micaela Navarro, Pablo E. Visconti, Mariano G. Buffone, A. Mutto, Darío Krapf,

Ovarian function and disorders

After ejaculation, mammalian sperm undergo a series of molecular events conducive to the acquisition of fertilizing competence. These events are collectively known as capacitation and involve acrosomal responsiveness and a vigorous sperm motility called hyperactivation. When mimicked in the laboratory, capacitating bovine sperm medium contains bicarbonate, calcium, albumin and heparin, among other components. In this study, we aimed at establishing a new capacitation protocol for bovine sperm, using calcium ionophore. Similar to our findings using mouse sperm, bovine sperm treated with Ca