Voltar
Artigo Acesso aberto Revisado por pares
BIBTEX RIS

The modified RNA base acp3U is an attachment site for N-glycans in glycoRNA

2024; Cell Press; Volume: 187; Issue: 19 Linguagem: Inglês

10.1016/j.cell.2024.07.044

ISSN

1097-4172

Autores

Yixuan Xie, Peiyuan Chai, Nicholas A. Till, Helena Hemberger, Charlotta G. Lebedenko, Jennifer Porat, Christopher Watkins, Reese M. Caldwell, Benson M. George, Jonathan Perr, Carolyn R. Bertozzi, Benjamin A. Garcia, Ryan A. Flynn,

Tópico(s)

RNA Interference and Gene Delivery

Resumo

GlycoRNA consists of RNAs modified with secretory N-glycans that are presented on the cell surface. Although previous work supported a covalent linkage between RNA and glycans, the direct chemical nature of the RNA-glycan connection was not described. Here, we develop a sensitive and scalable protocol to detect and characterize native glycoRNAs. Leveraging RNA-optimized periodate oxidation and aldehyde ligation (rPAL) and sequential window acquisition of all theoretical mass spectra (SWATH-MS), we identified the modified RNA base 3-(3-amino-3-carboxypropyl)uridine (acp

Referência(s)