
Innovative sustainable bioreactor-in-a-granule formulation of Trichoderma asperelloides
2024; Springer Science+Business Media; Volume: 108; Issue: 1 Linguagem: Inglês
10.1007/s00253-024-13261-9
ISSN1432-0614
AutoresLucas Guedes Silva, Renato Cintra Camargo, Gabriel Moura Mascarin, Camila Patrícia Favaro, Peterson S.O. Nunes, Cristiane S. Farinas, Cauê Ribeiro, W. Bettiol,
Tópico(s)Plant-Microbe Interactions and Immunity
ResumoThe advancement of fungal biocontrol agents depends on replacing cereal grains with low-cost agro-industrial byproducts for their economical mass production and development of stable formulations. We propose an innovative approach to develop a rice flour-based formulation of the beneficial biocontrol agent Trichoderma asperelloides CMAA1584 designed to simulate a micro-bioreactor within the concept of full biorefinery process, affording in situ conidiation, extended shelf-life, and effective control of Sclerotinia sclerotiorum, a devastating pathogen of several dicot agricultural crops worldwide. Rice flour is an inexpensive and underexplored byproduct derived from broken rice after milling, capable of sustaining high yields of conidial production through our optimized fermentation-formulation route. Conidial yield was mainly influenced by nitrogen content (0.1% w/w) added to the rice meal coupled with the fermentor type. Hydrolyzed yeast was the best nitrogen source yielding 2.6 × 10
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