Artigo Produção Nacional Revisado por pares

Metabolomics Analysis of (Geo)propolis from Brazilian Stingless Bees by FIA and UHPLC-HRMS (Orbitrap)

2024; Elsevier BV; Volume: 252; Linguagem: Inglês

10.1016/j.jpba.2024.116497

ISSN

1873-264X

Autores

João Fábio Turco, João Benhur Mokochinski, Yohandra Reyes Torres,

Tópico(s)

Healthcare and Venom Research

Resumo

Native stingless bees (Meliponini) from Brazil make (geo)propolis which is largely used in folk medicine, specially by indigenous and quilombos communities and beekeepers´ families but are progressively being recognized for their pharmacological activities. In this study, the ethanolic extracts of (geo)propolis (EEGs) from Melipona marginata, M. quadrifasciata, M. scutellaris, and Tetragonisca angustula were analysed by Flow injection analysis (FIA) and Ultra-high performance liquid chromatography (UHPLC) in a high resolution Orbitrap mass analyser (HRMS) to investigate and compare their chemical profile. Untargeted metabolomic approach based on UHPLC-HRMS experiments, and bioinformatic tools, allowed to annotate 59 compounds from diverse classes such as: flavonoids, phenolic compounds, sugars, terpenoids, and lipids. In addition, using multivariate tools and Flow injection- high resolution mass spectrometry (FIA-HRMS), it was possible to classify samples and identify marker ions related to the bee species or genus and to the geographical origin as a proof of concept.

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