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Establishment of an induced pluripotent cell line (ABCRIi001-A) from an elderly female for ageing research

2024; Elsevier BV; Volume: 81; Linguagem: Inglês

10.1016/j.scr.2024.103576

1876-7753

Filipa Esteves, David V.C. Brito, Ana Teresa Rajado, Nádia Silva, Joana Apolónio, Vânia Palma Roberto, Raquel P. Andrade, Sofia M. Calado, Maria Leonor Faleiro, Carlos A. Matos, Nuno Marques, Ana Marreiros, Hipólito Nzwalo, Sandra Pais, Isabel Palmeirim, Sónia Simãoa, Natércia Joaquim, Rui Miranda, António Pêgas, D. Raposo, Ana Paula Sardo, Inês M. Araújo, Clévio Nóbrega, Pedro Castelo‐Branco, José Bragança,

3D Printing in Biomedical Research

Human induced pluripotent stem cells (hiPSCs) hold promises to model and understand human diseases, including those associated with ageing. Here, we describe ABCRIi001-A, a hiPSC line generated from peripheral blood mononuclear cells (PBMCs) of a 79-year-old female enrolled in a study for development of an ageing score (ALFA Score). PBMCs were reprogrammed using three Sendai virus-based reprogramming vectors (hKOS, hc-Myc, and hKlf4). ABCRIi001-A showed normal morphology and karyotype, viral clearance, absence of genomic aberrations, and their pluripotency was confirmed by expression of pluripotency-related markers and their ability to differentiate into the three germ layers. ABCRIi001-A is valuable for ageing-related studies.