A high-performance thin-layer chromatography densitometric method for the separation of isomeric ceftriaxone in powder for injection formulation
2024; Nature Portfolio; Volume: 14; Issue: 1 Linguagem: Inglês
10.1038/s41598-024-77334-5
ISSN2045-2322
AutoresKissa W. Mwamwitwa, Bugusu Nyamweru, Mohamed A. Iwodyah, Gerald Sambu, Goodluck G. Nyondo, Emili Yondu, Adam M. Fimbo, Vicky Manyanga, Wilber Sabiiti, Danstan H. Shewiyo, Morven C. Shearer, Andrew D. Smith, Eliangiringa Kaale,
Tópico(s)Antibiotics Pharmacokinetics and Efficacy
ResumoThe aim of this study was to develop and validate a High-Performance Thin Layer Chromatographic (HPTLC) method for simultaneous determination of ceftriaxone and ceftriaxone e-isomer in powder for injection formulation. Ceftriaxone sodium injection is an antibiotic that used globally. It has Z/E geometrical conformation, in which ceftriaxone sodium and 3 ene-isomer have Z- conformation while (E)-isomer has E- conformation and the potential toxicity of ceftriaxone (E)-isomer has been reported. Thus, to safeguard the public health, a simple and easy to use, rapid and reliable method was developed for qualitative and quantitative determination of ceftriaxone sodium and its (E)-isomer. Samples were applied on HPTLC glass plates precoated with silica gel 60F254 by using Linomat semi-auto sampler. Separation was carried out using acetone, triethyl amine, water, chloroform and ethyl acetate as a mobile phase in different ratios. The R
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