Exploring the Inhibitory Potential of Phytosterols β-Sitosterol, Stigmasterol, and Campesterol on 5-Alpha Reductase Activity in the Human Prostate: An In Vitro and In Silico Approach
2024; Multidisciplinary Digital Publishing Institute; Volume: 13; Issue: 22 Linguagem: Inglês
10.3390/plants13223146
ISSN2223-7747
AutoresMădălina-Georgiana Buț, Amelia Tero-Vescan, Amalia Pușcaș, George Jîtcă, Gabriel Marc,
Tópico(s)Steroid Chemistry and Biochemistry
ResumoSteroidal 5α-reductase type 2 (S5αR2) is a key enzyme involved in the conversion of testosterone (TST) to dihydrotestosterone (DHT), a crucial process in the development of benign prostatic hyperplasia (BPH). Phytosterols (PSs), natural plant-derived compounds, have been proposed as potential inhibitors of S5αR2, but studies on their efficacy are limited. This study evaluates the inhibitory effects of three PSs (β-sitosterol, stigmasterol, and campesterol) on S5αR2 activity using a combined in vitro and in silico approach. The inhibitory activity of the respective PSs was assessed in vitro, by measuring TST and DHT, while molecular docking and dynamics explored PS interactions with S5αR2's active site. The in vitro tests indicated significantly higher IC
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