Artigo Acesso aberto Revisado por pares

Reduced Levels of miR-145-3p Drive Cell Cycle Progression in Advanced High-Grade Serous Ovarian Cancer

2024; Multidisciplinary Digital Publishing Institute; Volume: 13; Issue: 22 Linguagem: Inglês

10.3390/cells13221904

ISSN

2073-4409

Autores

Eva González-Cantó, Mariana Ribeiro Monteiro, Cristina Aghababyan, Ana Ferrero-Micó, Sergio Navarro-Serna, Maravillas Mellado-López, Sarai Tomás-Pérez, Juan Sandoval, Antoni Llueca, Alejandro Herreros‐Pomares, Juan Gilabert–Estellés, Vicente Pérez-García, Josep Marí‐Alexandre,

Tópico(s)

Cancer-related molecular mechanisms research

Resumo

High-grade serous ovarian cancer (HGSOC) is the most lethal form of gynecologic cancer, with limited treatment options and a poor prognosis. Epigenetic factors, such as microRNAs (miRNAs) and DNA methylation, play pivotal roles in cancer progression, yet their specific contributions to HGSOC remain insufficiently understood. In this study, we performed comprehensive high-throughput analyses to identify dysregulated miRNAs in HGSOC and investigate their epigenetic regulation. Analysis of tissue samples from advanced-stage HGSOC patients revealed 20 differentially expressed miRNAs, 11 of which were corroborated via RT-qPCR in patient samples and cancer cell lines. Among these, miR-145-3p was consistently downregulated post-neoadjuvant therapy and was able to distinguish tumoural from control tissues. Further investigation confirmed that DNA methylation controls

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