Artigo Revisado por pares

First Report of Anthracnose Caused by Colletotrichum sansevieriae on Dracaena trifasciata (Snake Plant) in Tennessee

2024; American Phytopathological Society; Linguagem: Inglês

10.1094/pdis-05-24-1049-pdn

ISSN

1943-7692

Autores

Marian R. Grskovich, Tom Creswell, John Bonkowski,

Tópico(s)

Yeasts and Rust Fungi Studies

Resumo

HomePlant DiseaseAhead of PrintFirst Report of Anthracnose Caused by Colletotrichum sansevieriae on Dracaena trifasciata (Snake Plant) in Tennessee PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of Anthracnose Caused by Colletotrichum sansevieriae on Dracaena trifasciata (Snake Plant) in TennesseeMarian Grskovich, Tom Creswell, and John BonkowskiMarian GrskovichDepartment of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, U.S.A., Tom Creswellhttps://orcid.org/0000-0002-3899-1829Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, U.S.A., and John Bonkowski†Corresponding author: J. Bonkowski; E-mail Address: [email protected]https://orcid.org/0000-0003-4626-4370Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, U.S.A.AffiliationsAuthors and Affiliations Marian Grskovich Tom Creswell John Bonkowski † Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, U.S.A. Published Online:3 Dec 2024https://doi.org/10.1094/PDIS-05-24-1049-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations Share on View article Dracaena trifasciata is a tropical plant native to Eastern Asia, commonly sold as a house plant throughout the United States. In February of 2022, D. trifasciata (unknown cultivar) with moderate damage from leaf spots were submitted to the Purdue University Plant and Pest Diagnostic Lab for diagnosis from a commercial greenhouse in Tennessee. Leaf lesions were sunken, circular, and brown and produced acervuli with single-celled conidia that were salmon colored when in masses. The pathogen was isolated from the plant by excising symptomatic leaf tissue, which was surface disinfected with a 0.825% sodium hypochlorite solution for 1 min, followed by two sterile water rinses. Tissue was plated onto quarter-strength potato dextrose agar (QPDA) and grown at 25°C for 14 days under fluorescent lights. Single-spore isolates were obtained by streaking conidia from three colonies on QPDA plates. Colonies produced gray aerial mycelia with salmon-orange colored, single-celled spores in acervuli, in concentric rings. A pink hue developed within the agar as the colony aged. Three single-spore isolates were grown on QPDA for 14 days before extracting DNA from mycelia. The DNA was amplified in the internal transcribed spacer (ITS5/4) region (White et al. 1990) and submitted for Sanger sequencing. Sequences from each isolate were aligned and then analyzed using the NCBI BLAST tool in which each isolate had 100% query coverage and >99.8% identity to the type specimen of Colletotrichum sansevieriae (NR_152313). The representative isolate 22-00076A was selected to conduct a phylogenetic analysis using the following gene regions: ITS (ITS5/4) (OQ999173), TUB2 (T1/Bt2b) (OR546282), ACT (ACT512F/783R) (OR546280), and CHS (CHS79F/345R) (OR555874) (Damm et al. 2012a, b; Weir et al. 2012; White et al. 1990). A Tamura-Nei neighbor-joining phylogenetic tree was generated using Geneious Prime (GraphPad Software, Boston, MA), which confirmed the fungus species identity. To confirm pathogenicity, six healthy D. trifasciata plants were wounded by piercing the leaf epidermis with a sterile scalpel. Then, a sterile pipette was used to place 0.5 ml of a spore suspension (7.1 × 106 conidia/ml) collected from 14-day-old cultures plus Polysorbate 20 (final concentration 0.4975%) on each wound. Six control plants were wounded and mock-inoculated with sterile deionized water. Each plant was incubated at approximately 25°C under approximately 12 h of natural sun and fluorescent lighting in a clear plastic bag to maintain 100% relative humidity. Inoculated plants began to develop water-soaked, necrotic leaf spots after 1 week. After 2 weeks, Colletotrichum sp. was reisolated on QPDA from each inoculated plant and determined to be morphologically identical to the isolate 24-00076A, thus confirming Koch's postulates. Pathogenicity testing was repeated once, yielding the same results. All mock-inoculated plants remained symptomless. Anthracnose of Dracaena sp. has been reported in Japan, Australia, Korea, Malaysia, India, Iran, Germany, and the United States in Florida and Ohio (Farr et al. 2021; Palmateer et al. 2012; Valero David et al. 2023). This pathogen has the potential to spread rapidly in high-humidity greenhouse conditions with overhead irrigation, which can lead to significant damage and make D. trifasciata unmarketable (Aldaoud et al. 2011). To our knowledge, this is the first report of C. sansevieriae causing anthracnose on D. trifasciata in Tennessee. The author(s) declare no conflict of interest.References:Aldaoud, R., et al. 2011. Australas. Plant Dis. Notes 6:60. https://doi.org/10.1007/s13314-011-0020-z CrossrefGoogle ScholarDamm, U., et al. 2012a. Stud. Mycol. 73:1. https://doi.org/10.3114/sim0002 CrossrefWeb of ScienceGoogle ScholarDamm, U., et al. 2012b. Stud. Mycol. 73:37. https://doi.org/10.3114/sim0010 CrossrefWeb of ScienceGoogle ScholarFarr, D. F., et al. 2021. United States National Fungus Collections Fungus-Host Dataset. Ag Data Commons. (retrieved 2024). https://fungi.ars.usda.gov/ Google ScholarPalmateer, A. J., et al. 2012. Plant Dis. 96:293. https://doi.org/10.1094/PDIS-06-11-0539 LinkWeb of ScienceGoogle ScholarValero David, G. E., et al. 2023. Plant Dis. 107:2252. https://doi.org/10.1094/PDIS-10-22-2476-PDN LinkGoogle ScholarWeir, B. S., et al. 2012. Stud. Mycol. 73:115. https://doi.org/10.3114/sim0011 CrossrefWeb of ScienceGoogle ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA. CrossrefGoogle Scholar Funding: Support was provided by NPDN–USDA National Institute of Food and Agriculture cooperative agreement 2022-37621-38278. The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Just PublishedSubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Published: 3 Dec 2024Accepted: 27 Sep 2024 Information© 2024 The American Phytopathological SocietyFundingNational Institute of Food and AgricultureGrant/Award Number: 2022-37621-38278KeywordsanthracnoseColletotrichum sp.fungitropical plantsThe author(s) declare no conflict of interest.PDF download

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