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Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches

2024; Cadmus Press; Volume: 65; Issue: 14 Linguagem: Inglês

10.1167/iovs.65.14.32

1552-5783

Ted S. Acott, Michael P. Fautsch, Weiming Mao, C. Ross Ethier, Alex S. Huang, Mary J. Kelley, Mini Aga, Sanjoy K. Bhattacharya, Terete Borrás, Diane E. Bovenkamp, Uttio Roy Chowdhury, Abbot F. Clark, Mohammed Dibas, Yiqin Du, Michael H. Elliott, Jennifer A. Faralli, Haiyan Gong, Samuel Herberg, Murray Johnstone, Paul L. Kaufman, Kate E. Keller, Ruth A. Kelly, David Križaj, Markus H. Kuehn, Hoi Lam Li, Raquel L. Lieberman, Shan C. Lin, Yutao Liu, Fiona McDonnell, Colleen M. McDowell, Gillian J. McLellan, Philip Mzyk, K. Saidas Nair, Darryl R. Overby, Donna M. Peters, Vijay Krishna Raghunathan, Ponugoti Vasantha Rao, Gavin W. Roddy, Najam A. Sharif, Myoung Sup Shim, Yang Sun, Benjamin R. Thomson, Carol B. Toris, Colin E. Willoughby, Hao F. Zhang, Thomas F. Freddo, Rudolf Fuchshofer, Kamisha R. Hill, Alireza Karimi, Krishnakumar Kizhatil, Casey C. Kopcyznski, Paloma B. Liton, Gaurang Patel, Michael Peng, Padmanabhan P. Pattabiraman, Ganesh Prasanna, Ester Reina‐Torres, E. Griffen Samples, John R. Samples, Cynthia Steel, Clemens Strohmaier, Preeti Subramanian, Chenna Kesavulu Sugali, Joseph M. Sherwood, Cydney A. Wong, Hannah Youngblood, Gulab Zode, Elizabeth White, W. Daniel Stamer,

Retinal Diseases and Treatments

Intraocular pressure (IOP) elevation is the primary risk factor and currently the main treatable factor for progression of glaucomatous optic neuropathy. In addition to direct clinical and living animal in vivo studies, ex vivo perfusion of anterior segments and whole eyes is a key technique for studying conventional outflow function as it is responsible for IOP regulation. We present well-tested experimental details, protocols, considerations, advantages, and limitations of several ex vivo model systems for studying IOP regulation. These include: (1) perfused whole globes, (2) stationary anterior segment organ culture, (3) perfused human anterior segment organ culture, (4) perfused animal anterior segment organ culture, (5) perfused human corneal rims, and (6) perfused human anterior segment wedges. These methods, with due consideration paid to their strengths and limitations, comprise a set of very strong tools for extending our understanding of IOP regulation.