NOTE ON THE PREPARATION OF HYDROXYPROLINE
1931; Elsevier BV; Volume: 90; Issue: 1 Linguagem: Inglês
10.1016/s0021-9258(18)76681-7
ISSN1083-351X
Autores Tópico(s)Analytical Chemistry and Chromatography
ResumoDuring the preparation of large quantities of proline in this laboratory a simple method for the isolation of hydroxyproline, as a by-product, has been developed.Town's (1) procedure, which depends upon the separation of the hydrolysis products of protein by means of the solubilities of their copper salts, was used for the isolation of proline.By this method the amino acids are divided into the following fractions; (Fraction 1) copper salts insoluble in water, (Fraction 2) copper salts soluble in water but insoluble in methyl alcohol, (Fraction 3) copper salts soluble in both water and methyl alcohol.Proline is isolated from Fraction 3. We have found that large quantities of hydroxyproline are also contained in this fraction.After removal of the copper, the hydroxyproline is readily separated from proline by its insolubility in absolute alcohol.It can then be isolated as the picrate from the alcohol-insoluble residue.The free hydroxyproline can be obtained by the decomposition of the picrate according to the procedure of Cox and King (2).Considerable quantities of the pure Z-hydroxyproline have been prepared in this laboratory by the above procedure.EXPERIMENTAL
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