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The efficacy and stability of five sanitizing agents challenged with reference microorganisms and clean area isolates

2011; UNIVERSIDADE EST.PAULISTA JÚLIO DE MESQUITA FILHO; Volume: 32; Issue: 2 Linguagem: Inglês

2179-443X

Catherine Simoes de Abreu, Felipe Rebello Lourenço, Terezinha de Jesus Andreoli Pinto,

Listeria monocytogenes in Food Safety

The antimicrobial activity of five sanitizing agents employed in clean areas designated for the pharmaceutical manufacturing of sterile products was tested against nine microorganisms, including four microorganisms from the clean area microbiota. The method consisted of challenging 5 mL of each sanitizing agent - 70% isopropyl alcohol, 0.4% LPH®, 1.16% hydrogen peroxide, 4% hydrogen peroxide, 1% Bioper® and 5% phenol - with 0.1mL each of concentrated suspensions (105 – 106 CFU/mL) of Staphylococcus aureus, Candida albicans, Corynebacterium sp., Micrococcus luteus, Escherichia coli, Aspergillus niger, Bacillus subtilis, Staphylococcus sp. and Bacillus sp. for 10 minutes, followed by serial dilutions and plating. The results demonstrated that the five agents were effective against S. aureus, C. albicans, Corynebacterium sp., and M. luteus. The same was true of E. coli, except that isopropyl alcohol showed low levels of inactivation. With A. niger, isopropyl alcohol, 0.4% LPH® and hydrogen peroxide were more effective and 5% phenol and 1% Bioper® less effective. 1% Bioper® and 4% hydrogen peroxide showed greater inactivation of Staphylococcus sp., Bacillus sp. and B. subtilis than the other agents. Against S. aureus, C. albicans, Corynebacterium sp. and M. luteus, 5% phenol showed similar activity to other agents, while with A. niger, B. subtilis, Staphylococcus sp. and Bacillus sp., it was similar to or less active than the other agents. It was demonstrated that two microorganisms from the clean area microbiota, Staphylococcus sp. and Bacillus sp., were the most difficult to eradicate, requiring more frequent application of hydrogen peroxide and 1% Bioper® than the other strains. Keywords: Biocides. Biocontrol. Disinfection. Pharmaceutical manufacturing. Sterilization. RESUMO Eficacia e estabilidade de cinco agentes sanitizantes avaliados frente a microrganismos de referencia e isolados de areas limpas O objetivo deste estudo e avaliar a atividade antimicrobiana de cinco agentes sanitizantes empregados em areas limpas construidas para a fabricacao de produtos farmaceuticos estereis contra nove microrganismos, incluindo quatro microrganismos oriundos da area limpa. A metodologia constituiu em desafiar 5 mL de cada agente sanitizante, alcool isopropilico 70%, LPH® 0,400%, peroxido de hidrogenio 1,160% e 4%, Bioper® 1% e fenol 5% com 0,1 mL de suspensao concentrada (105 – 106 UFC/mL) de Staphylococcus aureus, Candida albicans, Corynebacterium sp., Micrococcus luteus, Escherichia coli, Aspergillus niger, Bacillus subtilis, Staphylococcus sp. e Bacillus sp. individualmente por 10 minutos, seguido de diluicoes seriadas e plaqueamento. Os resultados demonstraram que os cinco agentes sanitizantes foram efetivos contra S. aureus, C. albicans, Corynebacterium sp., e M. luteus. Os mesmos resultados foram observados com E. coli, exceto para o alcool isopropilico, que demonstrou baixos niveis de inativacao. Contra A. niger, alcool isopropilico, 0.4% LPH® e peroxido de hidrogenio foram mais efetivos e fenol e Bioper® menos efetivos. Bioper® e peroxido de hidrogenio 4% demonstraram altos niveis de inativacao de Staphylococcus sp., Bacillus sp. e B. subtilis quando comparados com outros agentes. Fenol demonstrou atividade antimicrobiana similar aos outros agentes contra S. aureus, C. albicans, Corynebacterium sp. e M. luteus. Contra A. niger, B. subtilis, Staphylococcus sp. e Bacillus sp., a atividade antimicrobiana do fenol foi similar ou inferior a dos outros agentes. Foi demonstrado que os microrganismos isolados da area limpa, Staphylococcus sp. e Bacillus sp., foram os que apresentaram maior dificuldade para inativar, sendo necessaria a aplicacao de peroxido de hidrogenio e Bioper® , com maior frequencia. Palavras-chave: Biocidas. Biocontrole. Desinfeccao. Producao farmaceutica. Esterilizacao.